The Budr Mark Budr / Dna Dual-parameter Flow Cytometry Determination Of Tumor Potential Doubling Time Of The Study,

The study of methods for BUdR incorporation and BUdR/DNA bivariate flow cytometry (FCM) in determining Potential Doubling Time (T_pot)The T_pot, before radiotherapy of cancer may be indication for proliferation of tumour stem cell during treatment.The clinical result in head and neck squamous cell carcinomas(HN-SCC) shown: the pretreatment T_pot appears to be an important independent prognostic factor for local control of HN-SCC treated by primary radiotherapy ,it would be a useful parameters to guide to a choice fractionated schedules.The purpose of this study is to compare the method of BUdR incorporation in vivo and in vitro. HeLa cell line, xenograft of human nasopharngeal cancer (NPC) in nude mice and the biopsy or surgical specimen were studied for investigating the probability on in vitro BUdR incorporation instead of in vivo method. The following studies were done:1.The methods of preparation of BUdR, DNA denaturing, immunofluorescence staining, measurement by FCM, and the movement of labeled cell through the S-phase were studied on in vitro HeLa cell line.2. NPC xenografts in nude mice were used for T_pot measurement in vivo. The various methods preparing single cell suspension were compared. The influences by the volume of tumour and storage time of samples were analysed.3.Appropriate culture condition in vitro labeling method was obtained using human NPC xenografts in nude mice and surgical samples of rectum cancer, the tumour was cut into 1 -2mm³ pieces by a...