T Subsets I, Type Ii Cells In Hematopoietic Stem Cell Transplantation For The Study Of Change In The Proportion Of Recipients

The type I and type II T lymphocytes keep a balance in healthy people. The imbalance of type I and type II T lymphocyte determine the direction of immune response and inflammation. Different pathologic states can be charactered as a polarization to type I or type II response. From the animal aGVHD model, we learned that the type I T cells and its cytokines exert crucial function in aGVHD. While the percent of type I T cells in human aGVHD was not reported so far. Also in research of animals, the function of immune cells in donor was regulated by G-CSF and therefore influenced the immune of transplantation. But there were still controversial about the G-CSF function till now. In this work, we adopt technique of flow cytometry, detct the type I and type II T cell percentage in donor of hematogenic stem cell transplantation (HSCT) befor and after mobilized by G-CSF, and detct the percent change in receptors in the early stage post HSCT. We investigate the influence of G-CSF to the function of donor's immune cells and aGVHD as well as the association of type I and type II T cell percents with aGVHD. The works we have finished were described as below.1. The percent of type I and type II lymphocyte subsets in healthy adult and the influence of two different surface markers to the detection with flow cytometry. In this research, We have established effective laboratory technique...