.1. Investigated The Taxonomic Study Of The North Genus (kitasatosporia) In Category 2. Actinomycetes Ko-7132 From The Diversity Of 16s Rrna Restrictive Fragment

Traditional classification and identification for actinomycetes were according to their phenotypes. This method usually could not get affirmative conclusion, and sometime it could get wrong result. With the development of molecular biology and its broad utility in microbiology recent years, microbiologists apply gene information such as 16S rRNA or genomic deoxyribonucleic acid (DNA) as the criteria to classify bacteria in molecular level. It makes the microbiological taxonomy and identification more precise and reliable.The genus Kitasatosporia was found and named by Kitasato institute based on phenotypic characteristics in 1982. Recently someone proposed that the genus Kitasatosporia strains should be classified as a new species in the genus Streptomyces. To clarify the taxonomic status of Kitasatosporia from the molecular level, we amplify the almost all the 16S rRNA genes from some strains of genus Kitasatosporia and of genus Streptomyces by polymerase chain reaction and made restriction fragment length polymorphisms analyses through several endonucleases reactions and gel electrophoresis. The endonucleases Mbo I and EcoT14I digestion in Kitasatosporia genus strains produced peculiar fragments of 135 and 480 base pair, or 120 and 150 base pair, respectively. These fragments were not detected in the genus Streptomyces strains. Many different sequences between Kitasatosporia and Streptomyces were detected in the conserved region of the entire 16S rRNA gene regions. Base on these data and phenotypic differences it is proposed that the genus Kitasatosporia is a genus separated from Streptomyces in accord with accepted rules.In the course of screening antitumor antibiotics in Kitasato Institute, the strain KO-7132 was isolated with strong activity. We described here the results of the taxonomic studies on strain KO-7132, including morphological characteristics, cultural characteristics, physiological and biochemical characteristics, chemotaxonomic characteristics, sequencing the partial variable of region 16S rRNA...