Radish Gentiana Chemical Composition And Quality Control

Luoboqinjiao is the dried root of perennial herbage Phlomis medicinalis Diels which belongs to the genus Phlomis of family Labiate. It distributes in the west of Sichuan province and east of Xizang province in southwest China. According to the theories of Traditional Tibetan Medicine, it is cool in property and bitter in taste, and it has the functions of dispelling wind and clearing heat, relieving cough and reducing phlegm, promoting tissue regeneration and wound healing, and it is used as Tibetan Materia Medicine RADIX PHLOMII for symptoms and signs of common cold due to wind heat, cough with profuse sputum, pyogenic skin infection without healing for a long time, etc. Since there is little information about this crude drug as so far, its quality control items can't be established at present time. In order to study and develop Luoboqinjiao to provide theoretical proof and practical reference for clinical application, and to expatiate Luoboqinjiao used as Pangxiejia in various aspects, Luoboqinjiao was selected as the research object. Luoboqinjiao was studied systematically on the chemical constituents and quality control, and compared with Pangxiejia in various aspects, and approached simply on pharmacognosy and pharmacology at the same time. Our research results are as follows:1 To studies on chemical constituents from the roots of Luoboqinjiao. thirty-four compounds were isolated and repeatedly purified from 95% EtOH extracts of the roots of Luoboqinjiao and twenty-eight of them were identified by physicochemical properties and spectral analysis of UV, IR, MS(FAB-MS, ESI-MS, EI-MS and HR-MS), NMR(¹H-NMR and ¹³C-NMR), DEPT and 2DNMR(HSQC and HMBC). These compounds were identified as follows: phlomisoic acid(1), phlomisoside V(2), phlomisoside III(3), triacontanoic acid(4), triacontanoic acid, 2-(4-hydroxyphenyl)ethyl ester(5), phthalic acid, butyl isobutyl ester(6), palmitic acid(7), oleic acid(8), β-sitosterol(9), daucosterol(10), 5-hydroxy-7-methoxy-4,6-dimethylphthalide( 11 ),4-hydroxymethyl-2-furaldehyde( 12), 1 -dehydroxyshanzhigenin methyl ester(13), shanzhiside methyl ester(14), 6-O-acetyl-shanzhiside methyl ester(15), 8-O-acetyl-shanzhiside methyl ester(16), sesamoside(17), phloyoside II(18),...