Dingchuantang Declared Surrendering Decomposition Agent Against Respiratory Syncytial Virus Infection In Vitro And In Vivo Experimental Study

Respiratory syncytial virus is a kind of common etiological agent of acute lower respiratory tract infection in infants and children. There is mainly two clinical types that is respiratory syncytial virus pneumonia and bronchiolitis. They both characterized by breathing heavily, dyspnea, cough and even fever. Severe patients may have cardiac insufficiency. RSV infection severely harm infants and children' health. The late study had uncovered that there is close relationship between RSV infection and asthma. There isn't effective drug to cure RSV infection, but a lot of research study has obtained some achievement.To try to find out theory of curing RSV infection with traditional Chinese medicine(TCM ). We can find that RSV infection had attracted doctors of successive dynasties to study it. They study etiological factor, mechanism, symptem feature, therapeutic principle, transformation and prognosi of RSV infection. There are many famous prescription remained until now. Virus infection is only factor that causes influence to body in the early period, but the key is the pathologic progress of body's response to virus infection. Superiority of the TCM is rather to adjust immunological function than to kill virus. The focal point should be to study as a whole because Competence of TCM prescription is complexes. Study as a whole may well uncover the action of TCM prescription.TCM considers that major etiological factor of respiratory tract viral infection is affect of exogenous wind-heat seasonal pathogenic factors. Base pathogenesis of respiratory tract viral infection is phlegm-heat obstruction in the lung, so cooling and dispersing therapy can drive away stagnated exogenous pathogen. To lower QI and to dissipate sputum can adjust activities of QI and low adverse-rising lung-QI, which can relieve dyspnen. The study of Dingchuantang Decoction (DCT) to cure RSV infectious pneumonia is based on many years' clinical experience of doctor Wang. DCT from Many Prescription of Health Cultiveation is a famous prescription to cure children's cough and dyspnea caused by the exterior tightened by pathogenic wind-cold and accumulation phlegm-heat in the interior. The principal of the prescription is to disperse the inhibited lung-QI, low adverse-rising lung-QI and cool eliminating phlegm. The study is to analyze the rule of prescription and divide it into Xuan, Jiang and Qing disintegrant. We study their function on adjusting immunologic derangement caused by RSV infection.Experiment research on pharmacologic action of DCT and Xuan, Jiang, Qing disintegrant on Hela cell infected by RSV. DCT and Xuan, Jiang, Qing disintegrant of differeni density were applied to Hela infected with RSV , which turned out to having protective effect on Hela cell. The experiment contains two methods: one is given drugs before infection, the other is given drugs after infection. We concluded that DCT can inhibitetiological factor RSV in vitro through methods of Xuan and Qing, and prevention cell from infection was superior to direct inactivation of virus.Experiment research on serum pharmacologic action of DCT and Xuan, Jiang, Qing disintegrant on Hela cell infected by RSV. DCT and Xuan, Jiang, Qing herb-contained serum of different density were applied to Hela infected with RSV , which turned out to having protective effect on Hela cell. Conclution: Herb-contained serum of DCT and its disintegrant can inhibit RSV infection. Cell toxic action of herb-contained serum of Xuan was more serious than others, so we should be careful when using large dose of Ephedrae.Experiment research on therapeutic action of DCT and Xuan, Jiang, Qing disintegrant in mice model infected by RSV. Materials and methods: Balb/c mice were infected RSV-Long strains through dropping their nose with the virus to establish mice RSV pneumonia model. Studied inhibitive rate of lung indexes of DCT and its disintegrants through observing lung appearance and patliclog)' slice, examining RSV RNA by RT-PCR., using RT-PCR to test the expressions of RSV RNA, using ELISA to test the content of...