| More than 70% cerebrovascular diseases are caused by ischemia in clinic. The incident rate, death rate and mutilate rate of cerebral arterial thrombosis are very high. It has been one of mainly cause for death. Central nervous system, according to traditional concept, is except from immune system for the protection of blood brain barrier. With progresses in mechanism of brain damage, more accumulating evidences show that cytokines, adhesion molecules exist in brain tissues and cause inflammation. Inflammatory reaction plays a key role in brain damage. A new hot spot is coming in this field and more and more scholars pay attentions to it. The precondition of inflammation is abnormally adhesion between leucocytes and vascular endothelia cells in circulation.QINGKAILING parenteral solution is the improved dose derived from Angongniuhuang pills. The effects of QINGKAILING include removing fever, detoxication, expectorant and purging aperture. In the early days, it widespread in treating hyperpyrexia, infection and epidemic diseases, and it was extensively used to treat acute stroke since 1980s. This therapy is successful in clinic for the main mechanism partly coinciding with the new rising hypothesis of 'toxin damage brain veins'. The mechanism, the target points of QINGKAILING parenteral solution are not clear and more evidences are required to verify its effects in treating cerebrovascular diseases caused by ischemia. Thus, it is necessary to explore the molecular evens of 'toxin', 'meridian' and 'toxin damage brain veins'.This study was supported by a grant(G1999054404) from The National Program For Key Bisic Reasearch Projects(973)-'The effective components and mechanisms of QingKaiLing in depress ing cascade reaction of ischemic stroke'. Two time points, 12 hours and 24hours, are defined according to previous researches. For these progresses,this study focuses on the process of adhesion between endothelia cells and leucocytes. The level of neuronal and endothelia cell injury are evaluated and the expression levels of cytokines and adhesion molecules are detected after the cerebral ischemia model rat treated with QINGKAILING Our studies are performed as follows in five parts:Part one : The intervention effects of effective components of QINGKAILING on NE- κB - the initiation factor of vascular endothelia injury.The expression level of NF— κB are depressed by cholalic acid on endothelia after 12h ischemia. Jasminoidin obviously depress the expression level of NF—κB on both neur and endothelia after 12h and more stronger after 24h on neur. The depressed level of NF—κB was observed on endothelia in different periods in baicalin treating rats. The same effects on NF—κB are observed on endothelia, neur and glial cells when rats are treated with HeFang. In a word, these components can depress inflammation reaction by decreasing the expression of NF —κB. Part two : The intervention effects of effective components of QINGKAILING on expression of adhesion molecules in different period.Although the depress level is different in different time points in this part, the results show usthat the effective components of QINGKAILING can obviously decrease the level of E-selectin in cerebral tissues of MCAO rats except nacre. Nacre, HeFang and baicalin significantly reduced the expression of ICAM-1 after ischemia 12 hrs. After ischemia 24hrs, jasminoidin and cholalic acid begin to show their effects on depressing the expression of ICAM-1. The same tendency can be seen in the results of immunochemistry. These mechanisms contribute to the effects on depressing the primary and substance adhesion between leukocytes and endothelia.The results of immunochemistry show us that the expression levels of P-selectin are obviously decreased by nacre, jasminoidin, baicalin and HeFang on endothelia, and in a way, cholalic acid depress too. All components except cholalic acid depress the expression of PECAM-1 on endothelia, the strongest one is jasminoidin and HeFang takes the second place. Baicalin is more effective in the early period and... |
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