Hypothalamic Paraventricular Nucleus In The Ea Neiguan Anti-mechanism In Rabbits With Acute Myocardial Ischemia

Objective The incidence between acupoint and viscera is one of the most significant orientantion in the research on the essence of meridion. To proffer evidence to the study on the mechanism of electacupuncture on Neiguan curing AMI, the theme "stimulate on Neiguan acupoint can cure hear disease"which was verifed by massive clinic and study was chosen as the study content, the effect on MII by electacupuncture on Neiguan was observed, to quest the function of PVN and probe in the probable effector substance and the tract.Method After the ligation of the coronary artery,the effect of electricacupuncture on Neiguan including ST potential of ECG,the expression of interleukin -1 and β-endorphine in PVN and the monophasic action potential.the monophasic action potential durations and ultrastructure in the ischemial region is observed.Further more,the diversification of the effect after the electrolytic lesion on PVN or the microinjection NLX into PVN or the intrathecal injection AVPX in the C6 - T5 spinal segments were respectivly observed.Result 1.electricacupuncture on Neiguan can evidently reduce the preternatural increase of ST potential of ECG, the monophasic action potential and the monophasic action potential durations and meliorate the ultrastructure occuring after the ligation(p<0.05),and electricacupuncture 15 minite before the ligation is more effective than electricacupuncture just begining with the ligation(p<0.05);2. the electrolytic lesion on PVN causes temporarily slight AMI and can inhibit the effect of electricacupuncture obviously(p<0.05);3.the microinjection NLX into PVN evidently reduces the effect of electricacupuncture as soon as nearly no detective effect on the function of the normal rabbit heart;4.the intrathecal injection AVPX in the C6 - T5 spinal segments evidently reduces the effect of electricacupuncture as soon as nearly no detective effect on the function of the normal rabbit heart;Conclusion 1. electricacupuncture on Neiguan can signifisantly attennuate the MII,and electricacupuncture in advence is more effective ;2.PVN involed in the CNS thorough fare of the incidence between Neiguan and the heart;3. β -endorphine and interleukin -1 in PVN are active in the mechanism of electricacupuncture on Neiguan attenuating the MII;4. PVN-spinal Avp-ergic pathway is one of the probable efferent pathway in the machanism of electricacupuncture on Neiguan attenuating the MII;PVN takes an important role in the machanism of electricacupuncture on Neiguan attenuating the MII through numerous link of neuroimmunomodulation.