Naolisu Capsule To Improve The Experimental Study Of Vascular Dementia Rat Model Mental Retardation

Objective: Using Water maze , zymohistochemical method, immunohistochemical method as well as in situ hybridization, to discuss the effect and action mechanism of Nao Li Su (NLS) Capsule when treating the intelligent disorder of vascular dementia rats, through the stucy of influences of NLS Capsule on learning and memory abilities of vascular dementia rats, on hippocampal histomorphology , on the activity of nitricoxide synthase(NOS), Acid phosphatase (ACP), acetylchlinesterase (AchE) and Cytochrome Oxidase (CCO), on the transcription of nNOS positive cells and Never growth factor(NGF). Method: According to Olsson' s method, we replicated Vad rats model by blocking bilateral common carotid artery permanently and randomly classified into six groups, namely, model group, control group, NLS capsule high group, NLS capsule middle group, NLS capsule low dos group and pseudo-operation group(ten each group). The treatment lasted for twenty-one days. Water maze was used to test the learning and memory abilities of these Vod rats before and after making model and after treatment; We observed hippocampal histomorphology, the activity of ACP, CCO and NOS nNOS positive cells as well as NGFmRNA in virtue of light microscope, HE staining, zymohistochemical, immunohistochemical, genetic expression etc.Result: The learning and memory abilities of rats obviously improved (P<0. 05), the lose of hippocampal CA1 area pyramidal cells decreased apparantly; ACP, NOS activity andnNOS positive neuron decreased ; CCO, AchE activity increase ; NGFmRNA transcription is enhanced after trentment of high dosage NLS capsule and Dihydergotoxine. Conclusion: High dosage NLS capsule can improve the learning and memory ability ,increase CCO, AchE activity and decrease ACP, NOS activity . It indicated that NLS capsule can improve the cerebral ischemic state , protecte cerebral ischemic injury , accordingly to promote intelligence. Furthermore, NLS Capsule can inhibit the activity of nNOS via promoting the transcription of hippocampal NGFR, can regulate the balance between excitability and inhibitility of central nerves system, can increase the tolerance of neurocyte for hypoxia, can decline the lose of neuron and improve learning and memory abilities . And the effect of High dosage NLS Capsule is similar with that of Dihydergotoxine.