Bone Health Immortals Pill Osteoporosis Prevention Mechanism And Regulation Of Nitric Oxide Mechanisms

Gui-Lu-Er-Xian-Gao" is a traditional Chinese prescription, replenishing Qi and tonifying Yang, and nourishing Yin, base on my tutor clinic experience, replenishing Qi and nourishing kidney deficiency is the foundation principle , we develop the new traditional Chinese prescription "Jiang-Gu-Er-Xian- Wan" observed the therapeutic efficacy for osteoporosis and discuse mechanism, such as nitric oxide, and apoptosis. 1. The experiment of OVX mices. The postmenopausal model was established by cutting off the bilateral ovaries of the Kunming mices (10-11 weeks old). Baseline and sham-operated groups wer set up at the same time. The mices were either sham-operated or ovariectomized(OVX) besides one group was used as the base-line control. Three treated groups, received either 17-f3 estradiol or Raloxifene, or "Jiang-Gu-Er-Xian- Wan" Bone mineral density(BMD), bone biomechanicanics, biochemical were tested. The mice in OVX group got the following biological changes: the body weight increased with the fastest speed; the uteruses withered; the bone mineral density?and mineral content decreased greatly; biomechanical and histomorphological parameters lowered down, and turnover of bone accelerated compared with sham group. After treated with "Jiang-Gu-Er-Xian-Wan" the bone mineral density of the mice restored significantly and almost reach the level of sham operated group. Other parameters were also improved greatly, mineral content increased, biomechanical restored, and blood ALP were lowered down comparing with OVX group, calcium, phosphorus levels is in norml range. The uteruses were some proliferated, but at a lower degree, just like the Raloxifene not stimulate the uteruses, contrast to 17 -estrodial group, the uteruses appeared hyperplasia (p <0.01). 2. The experiment of OVX rats. Three-month-old female wistar rats were randomly divided into five groups. The rats were either sham-operated or ovariectomized(OVX) besides one group was used as the base-line control. Three treated groups, received either Premarin or low dose of herbs, or high dose of herbs. Bone mineral density(BMD), bone biomechanicanics, BGP, biochemical were tested. And the lumbar vertebrae section is stud by hybridization in situ of type I collagen, INOS, immunohistochemistry of iNOS, eNOS, and RT-PCR for iNOS. Rats in OVX group got the following changes: weight increased, BMD decreased, uterus withered, biomechanical and histomorphological parameters lowered down, and turnover of bone accelerated compared with sham group. After treated with 揓iang-Gu-Er-Xian-Wan? bone mineral density was restored greatly, biomechanical parameters were greatly improved, bone turnover was reduced. But uterus didn抰 appear hyperplasia, nor did blood calcium or blood phosphorus level increased. Hybridization in situ , immunohistochemistry and RT桺CR show the iNOS protein is expressed strongly in the OVX group, show the NO play a role in stimulate OC bone absorption. Premarin group iNOS is lighten in some degree, Jiang-Gu-Er-Xian-Wan?group the iNOS is barely detectable, but the eNOS expression is strength, the hybridization in situ of type I collagen is strongly expressed. 3. The experiment of HOS TE 85 cell strain. Recent work has shown that nitric oxide (NO) induction by nitric oxide synthase(NOS) is physiological mediator of bone cell function and demonstrate that it may be poss...