Effects Of Xiefei Zhuyin Decoction On Malignant Pleural Effusion Rats Of Tgf Beta1,Aqp1,Ctla-4and Pfp Ctla-4and Pfp

Objective:This experiment is to approach the mechanisms of xiefeizhuyin Decoction to MPE and the evaluation the curative effects of that, through the observation on the level of TGF betal,AQP1,CTLA-4and PFP in the pleural effusion and tissue on rat models of Malignant pleural effusion(MPE) and the survival rates of ratsMethod:100rats were divided into five groups randomly:blank group(A group),model group(A group), Chinese materia mediea group (C group), Mannatide injection group(D group) and Chinese materia mediea with Mannatide injection group(E group),20rats in each group.To set up80MPE rat models except blank group, were injected with EAC(Ehrliehs ascites carcinoma) in their pleural cavity.We observe the survival rates of rat models, detected the level of TGF betal and AQP1by immunohistoehemisty and the expression of CTLA-4and PFP by RT-PCR.Result:1. Compared with model group, median survival time and life extension rate in the C,D,E groups is longer(p<0.05), while that of C group and E group is longer than D group (p<0.05).2. Compared with model group, the pleural effusion volume of D groups (p<0.05) decreased,that of C and E group decreased Significantly (p<0.01).3. Observed under the light microscope, model group effusion smear,it is visible to see a large number of cancer cells to clump together with irregular shape. In the model group chest water sediment HE staining, there were a large number of cancer cells,with irregular shape and cytoplasmic serious imbalance. The pleural of the model group is thicken and serious damaged, there is a great deal of cancer cells with nuclei increased dense and clusters appear partially. About the above indexes, the treatment groups reduce the degree of that compared with model group. 4. The TGF betal content on pleural effusion of model group is higher than that of C and D groups (p<0.05),much higher than that of the E group(p<0.01). The TGF betal content on pleural tissue of model group is significantly higher than that of C,Dand E groups (p<0.01); D group is higher than that of the C group(p<0.05); C and D groups are much higher than that of the E group(p<0.01).5. The AQP1content on pleural effusion of model group is higher than that of C and D groups (p<0.05),much higher than that of the E group(p<0.01). C and D group are higher than E group on that (p<0.05). The AQP1content on pleural effusion and pleural tissue of model group is higher than that of C and D groups (p<0.05),much higher than that of the E group(p<0.01). D group is higher than E group on that (p<0.05).6. The CTLA-4content on pleural effusion and pleural tissue of model group is higher than that of C and D groups (p<0.05),much higher than that of the E group(p<0.01). D group is higher than E group on that (p<0.05). The CTLA-4content on pleural tissue of E group is lower than C and D group (p<0.05). The CTLA-4content on pleural tissue of C and D group is lower than D group (p<0.05).7. The PFP content on pleural effusion of model group is much lower than that of each treatment groups (p<0.01),while D group is lower than that of the C and E group (p<0.01). The PFP content on pleural tissue of model group is lower than that of C and D groups (p<0.05),much higher than that of the E group (p<0.01). The PFP content on pleural tissue of E group is higher than C and D groups(p<0.01).Conclusion:1. Xiefei Zhuyin decoction can extend the malignant pleural effusion rats on the survival period.2. Xiefei Zhuyin decoction reduce the pleural effusion on the malignant pleural effusion rats3. Xiefei Zhuyin decoction reduce the TGF beta, AQP1, CTLA-4 content on pleural effusion and pleural tissue of malignant pleural effusion rat.4. Xiefei Zhuyin decoction increase the PFP content on pleural effusion and pleural tissue of malignant pleural effusion rat, enhance the PFP in the activity, thus for the treatment of malignant pleural effusion.