Experimental Sudy Of Rabbit Mesenchymal Stem Cells Combind With PLGA Electrospinning Frame For Repairing Achilles Tendon Injury

Objective: To study the rabbit bone marrow mesenchymal stem cells (MSCs) combindwith PLGA electrospinning frame for repairing the Achilles tendon injuries. Methods:Made PLGA electrospinning membrane frame by using electrostatic spinning technology.To study PLGA electrospinning membrane frame with morphology observation andmeasurement. Disinfected the PLGA frame by five ways including high temperature andhigh pressure method, low temperature plasma, Co60illuminate, ultraviolet ray and ethanol.Used bacteria cultures to detect sterilizing effects and observed structure damage of PLGAframe; To harvested mesenchymal stem cells(MSCs) from rabbit bone marrow by usingdensity gradient centrifugation in vitro; Cultured P3generation of MSCs with PLGAelectrospinning frame;45adult Newzealand white rabbits were randomly divided into3groups: MSCs+PLGA frame group, PLGA frame group and control group. Each group had15r abbits. The central bundle of achilles of left hind legs of rabbit was cut off and suturedwith improved Kessler method. MSCs group used MSCs with PLGA electrospinningframe. PLGA frame group simply used PLGA electrospinning frame. The control groupsimply sutured only.5rabbits of each group were respectively put to death to obtainmaterial in2nd,4th,6th week. Achilles tendon healing were evaluated with histology andbiomechnaics analysis. Results: Fiber diameter of PLGA elctrospinning membrane framewas uniform. The structure was neat. The porosity was82.6±3.45%. Five kinds ofdisinfection way may achieve sterilization purposes. Co60illuminate and ultravioletradiation suitable for PLGA electrospinning membrane frame because of minimumdamaged to it. MSCs harvested by using gradient centrifugation separation got high purity,obvious biological characteristics and showed strong ability in vitro proliferation combinedwith PLGA electrospinning membrane frame. The histological and biomechanical evaluation tendon healing of MSCs group and PLGA frame group were better than that ofthe control group, with the surrounding tissue adhesion was lighter. Conclusion:Electrostatic spinning technology is a simple method for nanoscale fibers. MSCs harvestedby using gradient centrifugation separation got high purity, obvious biologicalcharacteristics and showed strong ability in vitro proliferation combined with PLGAelectrospinning membrane frame; MSCs combind with PLGA electricity spinning framecan promote the healing of endogenous rabbit achilles tendon injuries, reduce theexogenous healing intervention, reduce adhesion of tendons with surrounding tissue.