Expeirmental Research On CTGF,ILK In Pulmonary Ifbrosis Rats By Xiankekeli

Purposes:To make Pulmonary fibrosis rat models by Bleomycin, observe pathomorphism changes in these models effects on CTGF,ILK,CTGFmRNA,ILKmRNA in rat model'slungs, and discuss the prevention mechanism of Xiankekeli on Pulmonary fibrosis.Methods: Divide180rats into6groups at random, which are control group, modelgroup, prednisone group, high dose Xianke group(HD group), medium dose Xiankegroup(MD group) and low dose Xianke group(LD group), with30rats in each group. Theywere fed with common forage and were made into the models of Pulmonary fibrosis. On thesecond day after the models were successfully made, the rats in control group and modelgroup were administrated with Nacl, while the rats in prednisone group with prednisone, HDgroup, MD group and LD group with Xiankekeli10g/kg,5g/kg,2.5g/kg respectively. the7thday,14thday, and28thday respectively,measure Pulmonary fibrosis degrees in the lung dyedwith HE and Masson; measure the expression of CTGF,ILK in the lung by immunolhistochemistry, and CTGFmRNA,ILKmRNA by RT-PCR.Result:1The influence of Xiankekeli on lung quotientThe lung quotient of the rats in the model group is dramatically higher than the blankgroup at all time points, and there is remarkable statistical significance P<0.01.Compared between Xiankekeli HD, MD and LD groups and model group in lungquotient, there is no statistical significance on day7,14and28, P<0.05; compared betweenprednisone group and model group, there is remarkable statistical significance on day7,14and28, P<0.05；Compared between Xiankekeli HD and MD groups and prednisone group, there is thereis no statistical significance on day7,14and28, P>0.05; compared between Xiankekeli LDgroup and Xiankekeli HD and MD groups and prednisone group, there is remarkablestatistical significance, P<0.05.2The influence of Xiankekeli on pathomorphism of the rats with pulmonary fibrosis2.1AlveolitisAlveolitis integral in the rats in model group is significantly higher than blank group atall time points, and there is remarkable statistical significance, P<0.01；Compared between Xiankekeli HD, MD and LD groups, prednisone group and modelgroup on day7, there is remarkable statistical significance, P<0.05; Compared betweenXiankekeli HD, MD and LD groups, prednisone group and model group on day14and28, there is no remarkable statistical significance, P>0.05;Compared between Xiankekeli HD, MD and LD groups and prednisone group on day7and28, there is no remarkable statistical significance, P>0.05; Compared between XiankekeliHD, MD groups and prednisone group on day14, there is no remarkable statisticalsignificance, P>0.05; Compared between Xiankekeli LD groups and prednisone group on day14there is remarkable statistical significance, P<0.05;2.2Pulmonary fibrosisPulmonary fibrosis integral in the rats in model group is significantly higher than blankgroup at all time points, and there is remarkable statistical significance, P<0.01；Compared between Xiankekeli HD, MD and LD groups and prednisone group on day7,there is no remarkable statistical significance, P>0.05; compared between Xiankekeli HD,MD and LD groups, prednisone group and model group on day14and28, there is remarkablestatistical significance, P<0.05;Compared between Xiankekeli HD, MD and LD groups and prednisone group on day7,there is no remarkable statistical significance, P>0.05; compared between Xiankekeli HD,MD and LD groups and prednisone group on day14, there is no remarkable statisticalsignificance, P>0.05; compared between Xiankekeli HD, MD and LD groups, prednisonegroup and model group on day28, there is no remarkable statistical significance, P>0.05;compared between Xiankekeli LD group and prednisone group on day28, there is remarkablestatistical significance, P<0.05;3The influence of Xiankekeli on CTGF, CTGFmRNA expression of pulmonaryfibrosis rats' lung tissue3.1lung tissue CTGF expressionThere is increased CTGF expression of lung tissue in model group at all time points,which is significantly higher than that in blank group. There are large number of yellowparticles around lung tissue and alveolar wall, and there are dark yellow particles in focalvessels, part of inflammatory cell exuded from bronchus, focus and alveolar septum ininterstitial cell, and there is remarkable statistical significance, P<0.01;Compared between Xiankekeli HD, MD and LD groups and prednisone group andmodel group, there is CTGF positive expression in pulmonary interstice and around alveolarwall on day7,14and28, and there is remarkable statistical significance, P<0.05;Compared between Xiankekeli HD, MD groups and prednisone group on day7, there isno remarkable statistical significance, P>0.05; compared between Xiankekeli LD groups andprednisone group, and there is remarkable statistical significance, P<0.05; Compared between Xiankekeli HD groups and prednisone group on day28, there is noremarkable statistical significance, P>0.05; compared between Xiankekeli MD and LDgroups and prednisone group, there is remarkable statistical significance, P<0.05;3.2Lung tissue CTGFmRNA expressionThere is increased CTGFmRNA expression of lung tissue in model group at all timepoints, which is significantly higher than that in blank group. There is remarkable statisticalsignificance, P<0.01;Compared between Xiankekeli HD, MD and LD groups, prednisone group and modelgroup on day7,14and28, there is remarkable statistical significance, P<0.05;Compared between Xiankekeli HD, and MD groups and prednisone group on day7,there is no remarkable statistical significance, P>0.05; compared between Xiankekeli LDgroups and prednisone group, there is remarkable statistical significance, P<0.05;Compared between Xiankekeli HD groups and prednisone group on day14and28,there is no remarkable statistical significance, P>0.05; compared between Xiankekeli MDand LD groups and prednisone group, there is remarkable statistical significance, P<0.05;4The influence of Xiankekeli on CTGF, CTGFmRNA expression of pulmonaryfibrosis rats' lung tissue4.1Lung tissue ILK expressionThere is increased ILK expression of lung tissue in model group at all time points,which is significantly higher than that in blank group. There are large number of yellowparticles around lung tissue and alveolar wall, and there are dark yellow particles in focalvessels, part of inflammatory cell exuded from bronchus, focus and alveolar septum ininterstitial cell, and there is remarkable statistical significance, P<0.01;Compared between Xiankekeli HD, MD and LD groups and prednisone group andmodel group, there is ILK positive expression in pulmonary interstice and around alveolarwall on day7,14and28, and there is remarkable statistical significance, P<0.05;Compared between Xiankekeli HD, MD groups and prednisone group on day7, there isno remarkable statistical significance, P>0.05; compared between Xiankekeli LD groups andprednisone group, and there is remarkable statistical significance, P<0.05;Compared between Xiankekeli HD groups and prednisone group on day14and28, there is no remarkable statistical significance, P>0.05; compared between Xiankekeli MDand LD groups and prednisone group, there is remarkable statistical significance, P<0.05;4.2Lung tissue ILKmRNA expressionThere is increased ILKmRNA expression of lung tissue in model group at all time points,which is significantly higher than that in blank group. There is remarkable statisticalsignificance, P<0.01;Compared between Xiankekeli HD, MD and LD groups, prednisone group and modelgroup on day7,14and28, there is remarkable statistical significance, P<0.05;Compared between Xiankekeli HD, and MD groups and prednisone group on day7,there is no remarkable statistical significance, P>0.05; compared between Xiankekeli LDgroups and prednisone group, there is remarkable statistical significance, P<0.05;Compared between Xiankekeli HD groups and prednisone group on day14and28,there is no remarkable statistical significance, P>0.05; compared between Xiankekeli MDand LD groups and prednisone group, there is remarkable statistical significance, P<0.05;结论:Conclusion:1. Xiankekeli can exude through early stage restraint alveolar inflammatory factor, andconsequently lower the possibility of happening of advanced pulmonary fibrosis, which indicatesthat Xiankekeli has a very good effect on pulmonary fibrosis. There is no effective treatment onpulmonary fibrosis now, therefore, there is significance in conducting research on the effect andmechanism of Xiankekeli to pulmonary fibrosis.2. Xiankekeli HD group can restrain CTGF,ILK,CTGFmRNA,ILKmRNA, and theeffect is better than Xiankekeli MD group and LD group, but is equal to prednisone group.Therefore, Xiankekeli HD group has a very good effect on the prevention of pulmonary fibrosis.The effect of HD group is most significant which is equal to prednisone group and MD group issecondary and LD group is the least.3. Xiankekeli improve the abnormal deposition of cell epimatrix by restraining theCTGF,ILK,CTGFmRNA,ILKmRNA expression of pulmonary fibrosis rats' lung tissue, whichmight be one of the mechanism of preventing pulmonary fibrosis with Xiankekeli.