The Effects Of Buyang Huanwu Decoction On Proliferation Of Nerve Cell And Expressions Of TLR2,TLR4,MyD88,NF-κBp65in Rats With Cerebral Ischemia

Objectives:To explore the effects of Buyang Huanwu Decoction on proliferation of nerve cell and expressions of TLR2,TLR4,NF-κB, MyD88in rats with cerebral ischemia.Methods:Rat focal cerebral isehemia was induced by MCAO. Rats were randomly divided into following group including sham control, cerebral ischemia,cerebral ischemia with BYHW decoction.The group was divided into subgroups,animals in each subgroup were sacrificed in7,14or21day after operation. Neurological score of animals, the TTC calculating of their infarct area, to observe each group of animals the cell morphology in the hippocampus and cortex with HE staining, to observe BrdU labeling stem cells/progenitor cell proliferation using immunohistochemical staining and detecting the expression level of TLR2and TLR4.NF-κB and MyD88after cerebral ischemia using immunohistochemistry,the WB and RT-PCR were measured. Result:1) Compared with cerebral ischemia group BYHW decoction treatment can reduce the neurological deficit after focal cerebral ischemia, reduce the infarct size, improve the cellular morphology of the ischemic area and the surrounding tissue.2)Compared with cerebral ischemia group cerebral ischemia with BYHW decoction the BrdU positive cells increased significantly.3)Compared with the cerebral ischemia group at all time points each other neurological deficit and infarct area after focal cerebral ischemia had a trend of decreasing over time, morphology of ischemic area and the surrounding tissue are improved.4)Compared with the sham control, the number of BrdU positive cells was enhanced after ischemia in cerebral ischemia group.5)Compared with the sham group the expression of TLR2,TLR4,NF-κB and MvD88was increased after ischemia.6)Compared with the cerebral ischemia group, BYHW decoction treatment can lower the expression of TLR4, NF-κB and MyD88in ischemic brain tissue.7)Compared with the cerebral ischemia group BYHW decoction treatment can increase the expression of TLR2in ischemic brain tissue.Conclusion:1) Rats afer cerebral ischemia has self-repair mechanisms and can activate neural stem cell proliferation,that can compensate neurological deficit to a certain extent.2) BYHW decoction treatment has neuroprotective effects, and promotes proliferation of the neural stem cell after ischemia, which may play a role in its promotion of ischemic recovery of neurological function.3)TLR4/MyD88signaling pathway may be the mechanism of injury after cerebral ischemia.4)TLR2may be a protective factor after focal cerebral ischemia.5) BYHW decoction treatment can enhance the TLR2expression after cerebral ischemia, which may be one of mechanism of its neuroprotection and promoting nerve regeneration.6) BYHW decoction treatment can inhibit the expression of TLR4MyD88, NF-kappa B after cerebral ischemia,which may be a mechanism of its neuroprotective effects and promoting nerve regeneration through mitigating TLR4/of MyD88signaling pathway.