Laboratory And Clinical Research Of Shenshuguanxin Prescription In Anti Myocardial Ischemia

Objecti ve1Laboratory research:The aim of this study were studying the mechanism of anti-ischemic effects of shenshuguanxin prescription by studying its impact to cardiac function reserves and coronary microcirculation of the Experimental rat model of myocardial infarction.2Clinical research:Clinical evaluation of patients with stable angina pectoris receiving prescription treatment. Assess the impact of prescription on patients with stable angina pectoris, including the degree of angina relief, exercise tolerance, TCM clinical symptoms improvement and quality of life.Methods1Laboratory research:A total of60healthy SPF SD rats were randomly divided into six groups (n=10), normal control group (sham operation group), model control group (model group), the prescription of high dose group (high dose group), the prescription of medium-dose group (middle dose group), prescription low dose group (low dose group), compound Danshen Dripping Pills in the control group (Danshen Dripping Pill group), respectively. The AMI rat model of myocardial infarction underwent coronary artery ligation, comparing with the sham operation group with needle pass through without ligation. The interventions were began at3days after modeling and lasted for4weeks. Measured data of rats in each group, including hemodynamics, cardiac output, cardiac markers, infarct size, small and medium-sized blood vessels MVC value of infarct zone, infarct border zone of PECAM-1and VEGF immune group of expression.2Clinical research:The study used the method of double-blind, randomized, controlled. A total of120patients were involved and divided into treated group and control group, who were treated and diagnosed with stable angina pectoris from January2010to June2011, in clinics and wards of the Heart Center of the Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine (guangdong provcial hospital of TCM)(including the Hospital's headquarters, the Hospital's branch in Er Sha Island, Fangcun, Zhuhai and the University City). Two groups both accepted the norms of Western standard treatment, the treated group were treated with the prescription (produced by the Jiangyin Pharmaceutical Factory),1packet a day, the control group were treated with placebo formula (produced by the Jiangyin Pharmaceutical Factory),1packet a day, respectively. The intervention time was three months. Observed situations of these patient before, during and after treatment, including Angina pectoris attack, Chinese medicine symptom scoring, Seattle angina Quality of Life Scale.Results1Laboratory research:On comparison of MAP, UVSP, dp/dt max, dp/dt min and Cardiac output in high dose group and medium dose group of shenshuguanxin prescription and model group, there is statistical significant difference(P<0.05). And there is dose related expression. The shenzhuguanxin prescription group is better than model group and Danshen Dripping Pill group(P<0.05). There is decrease of infarct border zone MI in high dose group of shenzhuguanxin prescription group, and there is significant difference between high dose group of shenzhuguanxin prescription group and model group(P<0.05), and there are no significant differences between model group and the left of the groups(P>0.05). The increased expressions of MVC value of blood vessels in infarct zone, CD31of infarct border zone, and expression of VEGF is more than model group with significant difference(P<0.05) and dose relation. There is significant difference on comparison of MV^CD31> VEGF between shenzhuguanxin prescription group and Danshen Dripping Pill group (P<0.05).2Clinical research:on comparison of rate, level, persistence time, total dose of Nitroglycerin, percentage use of long action Mononitrate ester healer, percentage use of gastric mucosa protecting drug, Chinese medicine symptom scoring, and Seattle angina Quality of Uife Scale, the treated group is better than control group with significant differences(P<0.05).Conclusion:1. Shenzhuguanxin prescription has reliable curative effects on improving the reservation of hear function, micro-recirculation state of coronary artery, anti myocardial ischemia, and protecting cardiac muscle of MI rat model.2. Shenzhuguanxin prescription can decrease the symptoms of angina pectoris, improve the heart function, increase the toleration of patients' daily activities, protect the stomach intestine functions, improve the quality of life, and decrease the symptoms of Chinese medicine, with no infection of blood pressure and heat rate. It also has better compliance of patients, and no side effects.