Studies On Development And Material Base Of Soybean Total Flavonoids

Through the test,that active constituent of soybean has been found.We also gotoptimum technology by orthogonality, then we adopted macropore resin technology topurify it, and dynamic state adsorb investigation was taken into purification technology, thenwe built extraction pattern of Soybean total flavonoids.Using HLP to test content of genistein and Genistin in Soybean total flavonoids, andcarry out stability investigation, then build pharmacomodel which could react clinical effct,sieve active constituent and best effective dose. Animal acute toxicity testing and animallong term toxicity test show less toxic and side-effect of this medicine.By autonomic activities testing and pentobarbital sodium subthreshold dose synergismtesting, we observed Soybean total flavonoids, effect on center neural of mice, we alsoobserved effct on Cadiovascular and respiratory system of cats during narcotism.The resultshows that total effective part group has no obviously effet on mice center neural undernotified dose, likewise, no obviously effet on cats blood pressure, electrocardiogramandrespiratory system.Daidzein inhibited the transcription activation of NF-κB and the expressionalupregulation of pro-inflammation genes (IL-1β and IL-18) in LPS-stimulated murineRaw264.7macrophages. Likewise, the increase of protein poly(ADP-ribosyl)ation causedby LPS stimulation was also depressed by Daidzein treatment. Additionally, dual reportassay showed that the enhanced transcriptional activation of NF-κB caused byco-transfection of PARP-1was diminished by Daidzein. And immuno-precipitation assayshowed the enhanced binding of Rel A (p65) to PARP-1caused by LPS-stimulation was alsoblocked by Daidzein. The combined results form both dual report assay andimmuno-precipitation assay suggested Daidzein target to PARP-1activity, hence impact thetranscription of pro-inflammatory gene in immune response. Further in vitro inhibitory assayrevealed the depression of Daidzein on PARP-1activity might be through indirect signalingmechanisms more than acting as a competitor for PARP-1substrate under in vivo conditions.The combined results elucidated the mechanism of Daidzein to inhibit inflammation, andprompt it being a candidate to treat inflammatory diseases.