| In this paper, gas chromatography-mass spectrometry (GC-MS) analysis was performed for10samples of genuine producing areas in guangxi from curcuma kwangsiensis. The TIL profiles were evaluated by "Computer Aided Similarity Evaluation System"(developed by Research Center for Modernization of Chinese Medicine, Central South University), the fingerprint of essential oil from Curcuma kwangsiensis was established, and the characteristic peak area of the GC-MS fingerprint was acquired. The nasopharyngeal carcinoma CNE-2cells were treated with curcumol oil at different concentration12.5,25.0,50.0and100.0μg/ml), negative control (Ethanol medium) and positive control (5-Fu). The toxicity of Curcuma oil to CNE-2cells and the effect of inhibiting proliferation were detected by MTT method, the apoptosis was observed and analyzed by hoechst33342flourescence staning and flow cytometry, the expression of NF-κB was detected with western blotting; The characteristic peak area from10sample of genuine producing areas and inhibiting proliferation rate to CNE-2cells were analyzed by grey relational methods, the original data was standardized. At the premise of the standardized data, we calculated the absolute difference of reference series and comparison series, the correlation coefficient and correlation of the characteristic peaks and inhibiting proliferation rate to tumor cells. The results as follows:(1) Study on fingerprint of essential oil from Curcuma kwangsiensis:We separated52kinds of components in Curcuma kwangsiensis essential oil and identified39different compounds by GC-MS, mainly terpenoids and sesquiterpene derivatives. The20feature peaks in fingerprints of Curcuma kwangsiensi were identified over93%of the total area of the peaks, the resemblance was higher among10samples(>90%). The characteristic peak area from10sample of genuine producing areas had good separation, characteristics and uniqueness.(2) Study on pharmacodynamic of anti-tumor effect by Curcuma kwangsiensis essential oil:The Curcuma oil had strong toxicity to CNE-2cells in vitro, and was capable of significantly inhibiting proliferation of CNE-2, and the effect of inhibiting proliferation was dose-effect relationship. The Curcuma oil induced the apoptosis of NF-κB in the CNE-2cells, the molecular mechanisms may be related to the down regulated of the NF-κB protein level, and thus inhibit the proliferation of nasopharyngeal carcinoma CNE-2cells and promote apoptosis, and inhibit the growth of tumor cells.(3) Study on fingerprint's spectrum-effect from Curcuma kwangsiensis essential oil:The main anti-tumor active ingredient in curcumol oil was curcumol, curdione, β-elemene, germacrone and curzerenone. The curcumol was highly correlated with the effect of inhibitiing proliferation to nasopharyngeal carcinoma cell (relational garde>0.90). The relational grade was more than0.70between the curdione, β-elemene, germacrone, curzerenone and the effect of inhibiting proliferation to nasopharyngeal carcinoma cell. The relational grade was between0.60-0.80between other characteristic peaks represent the chemical composition and the effect of inhibitiing proliferation to nasopharyngeal carcinoma cell. According to the relational grade, the sequence of contribution to inhibiting proliferation to nasopharyngeal carcinoma cell was Curcumol> Curdione>β-Elemene> Germacrone> Curzerenone>a-Caryophyllene>δ-Selinene>β-Caryophyllene>β-Elemenone> a-Pinene>a-Caryophyllene> Eucalyptol> Camphor>2-Nonanol> Isoborneol>Curzerene>δ-Elemene> Camphene> D-Limonene> Borneol. The anti-tumor effect of Curcuma kwangsiensis was the result of combined effect of "effective component group".This study established the fingerprint of essential oil from Curcuma kwangsiensis, identified the effective component group represented by fingerprint contributed to pharmacodynamic, and provide a reference for establishing fingerprint of TCM's spectrum-effect relationship, TCM quality control and develop evaluation criteria of pharmacodynamics. |
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