| Objective:Efficacy of improvement sleep function material base of Suanzaoren decoction was determined preliminarily by using the method of traditional Chinese medicine serum pharmaceutical chemistry.Method:1.The content determination of the mangiferin and the Licorice glucoside in the Suanzaoren decoction:The qualification of the Suanzaoren decotion and ensue standardization of the experimental sample was determined by the contents of the mangiferin and the Licorice glucoside in the 257nm and 276nm by HPLC-UV with the mangiferin and the Licorice glucoside as the reference substance.2.To establish.the fringerprint of Suanzaorendecotion:The experiment complied with the technical regulations of The National Drug Supervision And Administration'The requirement of the research techniques of Traditional Chinese Medicine Injection fingerprint (temporary)', The chromatographic conditions of ten batch sample of Suanzaoren decotion was determined by HPLC-UV, and the fingerprint of HPLC-UV of the Suanzaorentang decotion was established.3.To confirm the Suanzaoren decotion freeze-dried the analysis and source of the mobiling composition in the blood of the rats:the wistar rats were irrigated for seven days with methanol removed the protein and prepared the cerebrospinal fluid. The mobiling composition of the cerebrospinal fluid was ensured and the source after the rats given medicine was affirmed in the the fingerprint of the Suanzaoren decotion.4.To analyse the Suanzaoren decotion freeze-dried the analysis and source of the mobiling composition in the blood of the rats:The wistar rats were irrigated for seven days, with methanol removed the protein and prepared the cerebrospinal fluid. The mobiling composition of the cerebrospinal fluid after the rats given the medicine was analysed by the fingerprint of the Suanzaorentang decotion condition.5.To preliminary confirm the efficacy material base of the improvement of the Suanzaorendecotion:in the condition of the fingerprint of the Suanzaoren freeze-dried, to analyse and contrast the external fingerprint, the mobiling composition fingerprint of the rats after giving medicine, the mobiling composition fingerprint in the cerebrospinal fluid preliminary confirmed the efficacy of material base of this prescription in the function of the improvement sleep6.To preminilary confirm endogenous substances in the cerebrospinal fluid:in the condition of the fingerprint chromatographic of Suanzaoren decotion reezed-dried, recording the HPLC of the neurotransmitter confirmed the endogenous substances in the cerebrosphinal.Result:1.In the Suanzaoren decoction the content determination of the mangiferin and the Licorice glucoside:the content determination of the mangiferin is 4.8091±0.1806mg/g,the content determination of the Licorice glucoside is 2.1059±0.0623mg/g.2.To establish the fringerprint of Suanzaoren decotion:Confirmed the fingerprint chromatographic conditions of Suanzaoren decotion freezed-dried-chromatographic column:waters company Symmetry C18 chromatographic column; elution system:acetonitrile-0.1% phosphoric acid water gradient elution; column temperature:30℃;detected wavelength:280nm; injection volume:10μl. The fingerprint for 10 batch samples was detemined, and confirmed 10 common peaks.3.To confirm the Suanzaoren decotion freeze-dried the analysis and source of the mobiling composition in the blood of the rats:in the chromatographic of the Suanzaoren decotion condition after giving seven days in the rats blood, it detected 6 mobiling contents, they are all metabolism products of producing by Suanzaoren decotion in the blood. Among them, mobiling contents 1,2 in the blood are all the metabolism products of the content of the Ziziphi spinosae, licorice in the blood;in the blood mobiling contents 3,4,5 are the metabolism products of the content of the licorice in the blood;the mobiling contents6 is the metabolism products of the contents of the Ziziphi spinosae and licorice in the blood;the mobiling content 4 don't appear in the serum chromatograms of the serum, it speculates that this mobiling contents don't product by any single herb, it is probable the new metabolism products of the new contents in the blood of the compound in the decoction process.4.To analyse the Suanzaoren decotion freeze-dried the analysis and source of the mobiling composition in the blood of the rats.After giving the medicine in the cerebrospinal fluid of the rats it appears that some endogenous material rises.5.To preliminary confirm the efficacy material base of the improvement of the Suanzaoren decotion:6 mobiling contents are probably the efficacy material base of the improvement of the Suanzaoren decotion in the serum after giving the medicine.6.To preminilary confirm endogenous substances in the cerebrospinal fluid:by contrast to the neurotransmitter,it detects that the endogenous substances are not anyone of the serotonin,5-hydroxyl ethyl indoles, norepinephrine, dopamine adenosine and urine glucoside what the composition is reminded to further research.Conclusion:1.The content of the mangiferin in the Suanzaoren decotion should be no less than 4.8091±0.1806mg/g, the content of the Licorice glucoside should be no less than 2.1259±0.0623mg/g.2.Establish the fringerprint of Suanzaoren decotion in the first, at 280nm, it detects 10 common peaks. They can fully reflect the existing state of the external components of the Suanzaorentang decotion.3.To confirm the Suanzaoren decotion freeze-dried the analysis and source of the mobiling composition in the blood of the rats:in the chromatographic of the Suanzaoren decotion condition after giving seven days in the rats blood, it detected 6 mobiling contents, they are all metabolism products of producing by Suanzaoren decotion in the blood. Among them, mobiling contentsl,2 in the blood are all the metabolism products of the content of the Ziziphi spinosae, licorice in the blood;in the blood mobiling contents 3,4,5 are the metabolism products of the content of the licorice in the blood;the mobiling contents6 is the metabolism products of the contents of the Ziziphi spinosae and licorice in the blood;the mobiling content 4 don't appear in the serum chromatograms of the serum, it speculates that this mobiling contents don't product by any single herb, it is probable the new metabolism products of the new contents in the blood of the compound in the decoction process.4.To analyse the Suanzaoren decotion freeze-dried the analysis and source of the mobiling composition in the blood of the rats.After giving the medicine in the cerebrospinal fluid of the rats it appears that some endogenous material rises.5.To preliminary confirm the efficacy material base of the improvement of the Suanzaoren decotion:6 mobiling contents are probably the efficacy material base of the improvement of the Suanzaoren decotion in the serum after giving the medicine.6.To preminilary confirm endogenous substances in the cerebrospinal fluid:by contrast to the neurotransmitter,it detects that the endogenous substances are not anyone of the serotonin,5-hydroxyl ethyl indoles, norepinephrine, dopamine adenosine and urine glucoside what the composition is reminded to further research. |
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