DanLongXingNao Fang Estrogenic Effects Of Treatment Of Senile Dementia Of The Mechanism Of Kidney

OBJECETIVE DanLongXingNao Fang to Vascular dementia (VD) treatment of senile kidney deficiency syndrome mechanism and clarify the different aspects of DanLongXingNao Fang treatment of VD rats treated with estrogen-like characteristics.METHOD The first week to 100 SD female big mouse to carry on the hypodermic injection D-galactose to create the old age model, the fourth week adds with the thyroxin piece suspension fills the stomach to create the kidney Yin deficient model, the seventh week divides into chastically the big mouse 4 groups:Sham-operation group, model+ physiological saline group, model+ DanLongXingNao Fang group, model+ estrogen group. In addition to separation of the sham group, only bilateral common carotid artery (CCA), the other rats in each group were repeated with CCA occlusion and reperfusion plus tails bleeding Methods VD rats. Clipping bilateral CCA, repeated three times, each time 10min, an interval of 10 min, while blood from the tip of the tail end cut 1cm 2ml, after normal feeding. After the technique 3d starts to treat. The sham-operation group normal diet potable water, does not make other processing; The model+ physiological saline group fills the stomach every day by 0.9% physiological saline; Model+ estrogen group inject once every three days of estradiol benzoate injection; Model+ DanLongXingnaoFang group uses traditional Chinese medicine DanLongXingnao Fang water decoctum to fill the stomach every day, treats 8W continuously. Treats 7W to carry on the water labyrinth to test each group of big mouse study memory function; After treating 8W, takes the big mouse hepatorenal, line of hepatorenal hydrometry; Draws blood carries on the estradiol (E2),the testosterone (T), the prostacyclin (PGI2), the bast nitrogen monoxide synzyme (eNOS) examines; Hippocampal brain tissue obtained as paraffin, HE staining, the histopathological examination, count the number of nerve cells in hippocampus of normal, the counting hippocampal normal nerve cell number, counts with the Tunel law perishes weakly the cell number; Takes the hippocampal brain organization, measured that the Bax, Bcl-2 protein expresses, measures the ERmRNA content with the RT-PCR technology.Results DanLongXingNao Fang group, estradiol group compared with the saline group:①the number of errors the water maze test, reduction of swimming time;②hippocampus of normal nerve cell counts increased significantly decreased apoptotic cells;③E2 levels by improving, No significant changes in the level of T, E2/T ratio increases;④eNOS, PGI2 content increased;⑤Bax protein expression was decreased, Bcl-2 protein expression increased;⑥ERmRNA expression of the ippocampus. DanLongXingNao Fang curative effect of group compared with the estradiol group, slightly better than the estradiol group. COUCLUSION The kidney and activating blood and collaterals DanLongXingNao Fang combined with the ER,like estrogen play a role in relaxing blood vessels and increase cerebral blood flow,direct protection of neuronal cells, thereby improving the rat model of dementia VD symptoms. This may be DanLongXingnao Fang VD Treatment of senile kidney deficiency one of the mechanisms.