Involvement Of Substance P In Inflammatory Pain And Jiaji Electroacupuncture Regulating Network Of Immune-Neuroendocrine Interactions

Objective: The aim of this study was to reveal the mechanism of involvement of central endogenous descending pain control system SP,NK-1R in Inflammatory pain and the regulaion of network of immune-neuroendocrine interactions by JIAJI electroacupuncture.Methods: Monoarthritis (MA) was induced by an injection of complete Freund's adjuvant (CFA). By using the molecular biological techniques,including Real-time PCR,immunochemistry,ELISA,the present study was designed to explore,(1) the changes of expression of SP,NK-1R in hypothalamus and spinal cord of MA rats and EA analgesia; (2) the changes of expression of TNF-αin hypothalamus and spinal cord of MA rats and EA analgesia; (3) the changes of expression of ratio of CD4+/CD8+ T cells in peripheral blood of MA rats and EA analgesia;Results:(1)The PWL were significantly prolonged in JIAJI EA group than routine EA group at 10th day and 14th day post-EA( P<0.05) ,but there was no significant difference between JIAJI EA group and JIAJI+routine EA group during the experiment( P=0.627, P=0.731, P=0.473) .(2)There was no significant difference between JIAJI EA group and Celebre group during the experiment( P =0.874,P =0.779,P =0.892), but which was further significantly enhanced by JIAJI EA +Celebre group at 10th day and 14th day post-EA( P =0.023 to P =0.044) .(3)The mRNA levels detected by Real-time PCR and the expression detected by immunohistochemistry of SP ,NK-1R in hypothalamus in JIAJI EA group were significantly increaced as compared to MA group(P<0.01),which was further significantly enhanced by JIAJI EA +Celebre group during the experiment( P<0.01) .(4) The mRNA levels detected by Real-time PCR and the expression detected by immunohistochemistry of SP ,NK-1R in spinal cord in JIAJI EA group were significantly decreaced as compared to MA group(P<0.01);Combination of EA with Celebre significantly enhanced the inhibitory effects(P<0.01).(5) The mRNA levels detected by Real-time PCR and the expression detected by ELISA in hypothalamus and spinal cord of TNF-αin JIAJI EA group were significantly decreaced as compared to MA group( P<0.01);Combination of EA with Celebre significantly enhanced the inhibitory effects(P<0.01).(6)The expression of ratio of CD4+/CD8+ T cells in peripheral blood in JIAJI EA group were significantly decreaced as compared to MA group(P<0.01);Combination of EA with Celebre significantly enhanced the inhibitory effects( P<0.01) .Conclusion:(1)Comulative EA has potent analgesia effect on inflammatory pain and JIAJI EA is more effectively than routine EA. Combination of EA with Celebre significantly enhanced the inhibitory effects on hyperalgesia.(2) These results show that JIAJI EA could regulate levels of SP,NK-1R and TNF-αin hypothalamus and spinal cord,suggesting that central endogenous descending pain control system SP,NK-1R and TNF-αplayed an important role in JIAJI electroacupuncture regulaing network of immune-neuroendocrine interactions.