Analysis On The Physiological And Biochemical Characteristics Of The Different Combing Ability Rice Restoring Lines And Their F₁s.

Physiological and biochemical characteristics of the different combining ability restoring lines in rice were compared using F1s combined by restoring lines F89, F196, F177, F184, F175 (the restoring lines were the generations of R402×G37 and their agronomic characteristics were near to each other and stable) and Minghui63 with sterile lines Zhengshan97A, Jing23A, K17eA, ZhongjiuA and XueqingzaoA, such as characteristics of the anther and pollen, differentiation and retrogression of branch and spikelet, absorption efficiency of ions and its kinetics, root ability and its declined properties after heading, flag leaf photosynthetic and senescence characteristics, translocation of assimilates of different combining ability restoring lines The main results were as follows:The orders of the restoring lines for the generally combining ability (GCA) of yield per plant were:F89 (4.48)>F196 (2.83)>F177 (0.16)>F184 (-1.66)>F175 (-2.34) >Minghui63 (-3.46)The important traits for the high combining ability restoring lines were higher potassium absorbing ability of young root, bigger panicle model, less retrogressed spikelets, and bigger pollen, more pollen amount and higher pollen activity, higher yield per plant, higher rETR(relatively electron transfer rate) and Fv/Fm after the full expanding of the flag leaf, lower activity for the SPS, lower daily dynamic of the flag leaf starch content at heading. lower variations of dry weight per spikelets, lower Fv/Fm and Y (Ⅱ), higher Y(NO), higher SOD activity and the MDA content after heading, along with derease of chlorophyll content of flag leaf and the photosynthetic rate after heading, lower photosynthetic rate, stomatal conductance at grain filling stage, plant no maintaining leaf green and better leaf coloure change.Correlations between the combining ability yield per plant and nitrogen and phosphate uptake kinetics parameter during seedling stage, size of pollen, the root activity, spikelet root exudate at heading, the root activity declined value 30 days after heading, chlorophyll fluorescence parameter Y (Ⅱ) after the full expanding of the flag leaf and ETR at heading, the MDA content of flag leaf, the leaf surface temperature and temperature discrepancy between air after heading, flag leaf inorganic phosphate content and its daily dynamic, flag leaf sucrose content and its daily dynamic, flag leaf starch content, the activity for the RuBPCase at heading, chlorophyll content of flag leaf and net photosynthesis rate, stomatal conductance, intercellular CO2 concentration from the full expanding of the flag leaf to 20 days after heading were insignificant.The important traits for the F1s of the high combining ability restoring lines were lower potassium absorbing ability of young root, lower chlorophyll content of flag leaf after the full expanding of the flag leaf, negative significant at 0.05 level correlations between flag leaf inorganic phosphate content and flag leaf sucrose content, higher flag leaf sucrose content in 12:30 at heading, bigger panicle model, more spikelets, less pollen amount, small anther, lower chlorophyll fluorescence parameter Fo,Fm,F,Fm'at heading, along with derease of chlorophyll content of flag leaf and the photosynthetic rate after heading, lower photosynthetic rate and variations of dry weight per spikelets at grain filling stage.not maintain green and easy to change coloure.