Effects And Mechanisms Of Antisense Oligonucleotides Of Connective Tissue Growth Factor On The Apoptosis Of Hypertrophic Scar Fibroblast

Objective:To explore the effects and mechanisms of antisense oligonucleotides of connective tissue growth factor on the apoptosis of human hypertrophic scar fibroblast. Methods:MTT shade selection experiment,laser confocal microscope and spectro-fluorescence technique were employed to learn the feasibility of CTGF ASODN transferring into HSF by TransFectin. The flow cytometer and DNA Ladder experiment were employed to detect the apoptotic of HSF and the expression of Fas. SA-β-Gal experiment was employed to detect the cell senescence. The CTGF,Fas and Bcl-2 mRNA index (RI) of HSF was assessed by reverse transcription polymerase chain reaction method ( RT-PCR). Results:Compared to the control group, antisense oligonucleotides could restrain the expression of connective tissue growth factor gene, promote apoptosis and cell senescence , enhance the expression of Fas gene. Conclusion:CTGF ASODN could promote apoptosis of HSF in vitro. The mechanisms were concerned with up-regulation Fas gene.