| Stress means unspecific response of organism characteres with function reinforcement of sympathetic nerve– adrenal medulla and hypophysis-adrenal gland cortex stimulated fiercenessfully. Stimulated by intensive physical or psychological stimulation factor, organism appears specific response directly associate with the stimulation factor, as well a group of unspecific response that has no direct relationship with that. Whatever the character of stimulation factor, the unspecific response is similar. Though immunological function is reinforced or inhibited by various kinds of neuroendocrine changes caused by stress. Their balance controls the immune state. Persistent stress usually results in immunosuppression due to glucocorticoid and catecholamine. Strong psychophysical stress or longtime chronic psychophysical stress does great harm to human health, which causes emotional abnormalities, excitatory sympathetic neural activity, gastrointestinal injury, low response of immunity and abnormality of neuroendocrine function as people. Stressing incidence has been considered one of the most important contributors to those functional diseases. Modem medical patern is the integrity of biological, psychological and social aspects. So far, no standard animal model has been developed, which limits the research in the efect of psychological factor on stress. Therefore, development of a psychological animal stress model is not only very helpful for management of psychological diseases, but also provides essential information for prevention or relief of stress. The studies show that gastroenterology system and immune system are the most seriously damaged by stress attack.It is well kown serious stress lead cellare immune function reducing and humoral immunity strengthening,as a result, Th1 turning into Th2. Gastroenterological tube is not only the place where digesting and absorb nourish matter,but also is the biggest immune organ in the body. If the gastroenterological tube immunity is decreased by every damage factor, The microorganism such as bacteria,virus and parasite well invade the body. It will cause gastroenterology system or the whole body pathological chages. The level of gastroenterological tube secretory immunoglobulin A (sIgA) will show the level of gastroenterological immunity.The question of if there is relationship between apoptosis and gastroenterological damage attacked by stress is still well known.It is reseach hot spot.Modern war notable characteristion is high technique, high intersity and high massacre power. The participater must undertake strong physiological and physical stress under modern battlefield environment. Gastroenterological tube is always attacked by stress, as the results lead to obstaction of digesting, obsorbing,secreting,moving,immunity and mucosal barrier. Stress ulcer, mucosal barrier obstacte and gastrointestinal motility weak are stress leading pathological results,which are difficult point and main point to save the sick and wounded.Soving the difficults can not only announce the principte of disease based of stress bult also improve the level of field medicine cure and prevent.Wistar Rats were our experiment objects,which were used to prepared water immersion restraint stress (WRS)models.The systematic immunity such as the levels of serum TNF-α,IL-6,IL-2 were tested by ELISA. The level of sIgA and Cortisol(Cor) were studed. The apoptosis index was test by Tunel. The expression of Bcl-2 and Bax was studied by immunohistochemistry. The correlation between this data change and psychological stress was studied.Accoring the research,we will work out the scheme to prevent gastroenterological function disorder base of modern war wound.Methods48 Wistar Rats were randomly divided into three groups as A group,B group and C group, the number of every group was 16. A group given suit swimming exercise and water immersion restraint stress . B group given suit swimming exercise nly. C group given water immersion restraint stress only. Suit swimming exercise meas free swimming 30 min 2/d,×5d. After suitswimming exercise, the rats of A and C group were given water immersion restraint stress after light anaesthesia, 90 min.After WRS experiment, we kill all rats and gain the vein blood and serum after centrifuge. The levels of serum TNF-α,IL-6,IL-2 were tested by ELISA. The level of sIgA and Cortisol(Cor) were studed. The levels of superoxide dismutase(SOD) and molondialdehyde (MDA) were tested by biochemical methods. The apoptosis index was test by Tunel. The expression of Bcl-2 and Bax was studied by immunohistochemistry. The correlation between this data change and psychological stress was studied.Results1. After model experiment, all rat were survival. There was not bleed and ulcer in the stomach in either rat of A and B group. Only four from sixteen rats of C group were found bleed and ulcer in the stomach. There three, one and six rat were found pale intestine and small bleed.2. The levels of serum TNF-α,IL-6,IL-2 of the rats of A and B group were higher than that of C group (P<0.05). There was significantly difference between three group either(P>0.05).3. The sIgA levels of the rats of A and B group were higher than that of C group (P<0.05).Data was 36.3±3.6mg/L and 39.1±4.0mg/L VS 27.1±2.8mg/L.4. The level of serum SOD of the rats of A and B group were higher than that of C group (P<0.05). The level of serum MDA of the rats of A and B group were lower than that of C group (P<0.05).5. Acute inflammation was observed in gastroenterological mucosa in the rats of three group.6.Tunel showed there was significant difference between three group in the apoptosis index(P<0.05). The expression of Bcl-2 and Bax of A and C group was significant different than that of C group. AI was direct relation with expression of Bcl-2 and inverse relation with expression of Bax.ConclusionsSuit swimming exercise can improve the rats attacked by stress in their systematic immunity and gastroenterological mucosal immunity. It can improve the rats'elinity ability to radical scavenger. The rats resisted the apoptosis caused by stress by way of promoting expression of bcl-2 and decreasing expression of Bax.Suit physiological and physical exercise can reduce apoptosis caused by stress.
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