A Preliminary Study On The Mechanism Of Protective Effects Of Deferoxamine In Brain Ischemic Reperfusion Injury

Ischemic preconditioning has precisely protective effects on brain, which works not noly inhuman but in animals. But the mechanism of the measurement is unclear. Deferoxamine(DFX),an iron chelator can induce tolerance against cerebral ischemia in humans and rats.Hypoxia-inducible transcription factor 1(HIF-1) is a master switch of the transcriptional responseto hypoxia and protected against hypoxia and ischemia. Erythropoietin (EPO) is a potentneuron-protectant in vivo and in vitro. EPO is the downstream target genes of HIF-1. Our studyfocus on the mechanisms of the preconditioning induced by DFX and the protection phenomenaof DFX against focal cerebral ischemia. Rats and cultured neurons and astrocytes which weredeprived of oxygen and glucose(OGD) by DFX, and then followed by oxygen-glucose re-supply.We investigated the effects (protection or damage)of DFX and whether DFX-induced tolerance isdependent on protein synthesis and correlates to HIF-1 and EPO. we can conclused: the DFXcan induce tolerance against cerebral ischemia in rats,cultured neurons and astrocytes, which mayresult from DFX upregulation the expresstion of HIF-1 a and its target gene EPO.