In Vitro Differentiation Of Bone Marrow Stromal Cells And Its Effect On Repair Of Full-thickness Articular Cartilage Defects

1. Investigation of the growth characteristics of cultured rabbit marrow stromal cellsAim: To investigate the growth characteristics of cultured rabbit marrow stromal cells (MSC), which will serve as the experimental basis for using MSC as the functional cells in bone/cartilage tissue engineering. Methods: Bone marrow was aspirated in the proximal part of tibia. MSC were separated for primary culture in which the CFU-F formed. Subculture was done for 5 passages consecutively, the initial cell density being 1 ×l0~4cells/cm~2. The following data were obtained: growth curve, cell doubling time, division rate and adhensive rate. Results: The growth curves of passage 1-5 were very similar. The doubling time of the third passage was 24h. The highest division rate was 19 ‰. 95% of the subcultured cells were adhesive in 12h. Conclusion: MSC show stable growth in vitro and relatively rapid proliferation and they easily survive the subculture under the present culture conditions. MSC may be used for articular cartilage repair by autogeneous cartilage engineering.2. In vitro study of Differentiation of bone marrow stromal cells to chondrocyteAim: The bone marrow stromal cell (MSC) is a differentiationally heterogenous cell group, including reticular cells, adipocytes, fibroblasts, osteogenic cells and stem cells,...