The Effect Of High Glucose And ROSIiglitazone On Apoptosis And Integrin-beta₁ Expression Of Proximal Tubular Cells

Department of nephrology, West China Hospital of Sichuan University.Peroxisome proliferator-activated receptor-γ (PPAR-γ) are ligand-activated transcription factors that regulate cell growth, inflammation, lipid metabolism, and insulin sensitivity. PPAR-γ in the human kidney has been described. However, the role of PPAR-ligand in proximal tubular cells with respect to cell adhere and apoptosis in diabetic nephropathy is largely unknown. We evaluated the effect of high (30 mM) D-glucose, thiazolidinedione Rosiiglitazone (5 μM and 10μM) on Integrin-β1 expression and apoptosis in the proximal tubular cells. Method: 1: proximal tubular cells were divided into 6 groups: Control group, Normal glucose group, high glucose group, high glucose with Rosiiglitazone 5μM group, high Glucose with Rosiiglitazone 10 μM group and Rosiiglitazone 10μM group. The cells were cultured for 48hrs, integrin β₁ and intrgrin β₁ mRNA were detected by Western Blot and RT-PCR. 2: The apoptosis was evaluated by flow cytometry after the cells were cultured for 24, 48 , 72hrs . Result: The expression of integrin β₁ is increased in high glucose group than control group and normal...