The Full Length Cloning And Preliminary Functional Analysis Of A Novel Human Gene PCIA1

Objectives: Completed in 2003, the Human Genome Project (HGP) identified all the approximately 20,000-25,000 genes in human DNA. These data mean that human whole genes are much smaller than we expected before, and a large amount of these genes are genes of unknown function. Our further work is to identify these genes and their function. In our previous study, we identified part of a novel human cDNA using SEREX technique. In order to study the possible function of this gene, full-length cDNA must be obtained and bioinformatic analysis as well as fundermental functional analysis should be performed.Methods: We screened part of the gene sequence of a new gene used SEREX (Serological analysis of recombinant cDNA expression library) approach. The full-length cDNA of the novel gene was obtained by RACE technology and then identified by RT-PCR. Various bioinformatic methods were used to analyze and predict the feature and possible functions of the novel gene. The tissue expression of the novel gene was identified by RT-PCR and the subcellular localization was identified by GFP fused protein as well as immunohistochemistry. The prokaryotic protein and polyclonal antibody were prepared. We further cloned it into eukaryotic expression vector and...