Repairing The Defect Of Urethral Extracellular Matrix Seeding With Cells: An Experimental Study In Rabbit Model

Objective: To construct engineering urethral tissue in vitro using technique of Tissue Engineering and stem cells for repairing the urethral defect of a rabbit model. Looking for better ways for construction of urethra in vitro, and comparing the different between involve seeded matrices and unseeded matrices in the repairing process. To elucidate the destiny of the seeding cells in vivo and the possibility of using BMSC with fibrin glue as a bulking agent to treat stress incontinence and vesico-ureteral reflux.Methods: (1) Bladder urothelium, bladder smooth muscle cells and bone marrow mesenchymal stem cells (BMSC) were choose as seed cells, primary culture and purify system were build to proliferate the seed cells. Two culture system (method 1 and method 2) were built for urothelium and one was choose to culture them in posterior studies. The proliferation characteristic of these cells were studied using cell counting and MTT Cell Proliferation Assay. To modify the BMSC, a liposome based plasmid transfection system was used to transfect BMSC, and its proliferation condition was studied after transfection. (2)Seeding BMSC and urothelium on the BECM and SIS was...