Construction Of LongSAGE Libraries Of Mouse Endometrium And Sage Analysis Of Differential Gene Expression During Pre-Implantation And Implantation

Embryo implantation is one of the earliest events in reproduction of humans and mammals that determines whether pregnancy will develop successfully. Successiful implantation depends on invasive blastocyst, acceptive endometrium and their synchronization.This process primarily begins with an intimate cross-talk between the embryo and the uterus. Numerous studies imply that the process of embryo implantation requires participation of numerous molecular and cellular factors, including endocrine, paracrine, autocrine, and juxtacrine modulators, to induce multifaceted cellular signaling pathways that, presumably, are regulated in a spatiotemporal manner both in the embryo and in the uterus. Initiation of embryo implantation occurs at the blastocyst stage and there is a short defined'window'of uterine receptivity for implantation, the so-called"implantation window". The window of implantation in mice only lasts for a period of a few hours on Day 5 of pregnancy. Implantation in all mammals involves shedding of the zona pellucida, followed by orientation, apposition, attachment and adhesion of the blastocyst to the endometrium. And blastocysts can only implant once they have been activated by uterine factor(s) that are regulated by ovarian steroid hormones. The regulation of early embryo development and the detailed molecular mechanism of implantation by which embryos invade into the uterus remains poorly understood, due to the large number of genes and the complexity of the...