Effect Of Rosiglitazone On Vascular Restenosis And Its Mechanism

BACKGROUND AND OBJECTIVEThe prevention and treatment of vascular restenosis after percutaneous coronary intervention(PCI) are a hot and difficult problem in the field of cardiovasology. The essence of restenosis is an excess repair after vascular injury. Proliferation and migration of vascular smooth muscle cells(VSMCs) from the media to the intima as well as accumulation of extracellular matrix are crucial pathogenic events in the progression of restenosis. The purpose of this study are to determine the effects of peroxisome proliferator-activated receptorγ(PPARγ) ligand—rosiglitazone(RSG) on VSMCs Proliferation and migration in vitro, and to investigate the effects of RSG on neointimal formation in the balloon injury model of rat carotid artery, and to explore the involved potential molecular mechanisms. All of these will be helpful to provide a new idea for the prevention and treatment of vascular restenosis after PCI.METHODS1. The primary and transfer culture were done by modified tissue-piece inoculation and trypsin digestion respectively. The cells were purified by several methods, including natural passage transfer, mechanical treatment and differential attachment. The cultured cells were identified by morphological characteristics and immunocytochemistry with antibody toα-actin.2. The fifth passage purified cells were harvested for the following experiments. Three methods were applied to explore the effects of RSG(1～10umol/L) on VSMCs proliferation induced by PDGF-BB or bFGF from different respects. MTT assay was used to quantitate the cell proliferating viability. Flow cytometry was preformed to...