Effect Of Adenovirus-mediated Gene Transfer Of Human Interleulin-10 On Ischemic Reperfusion Injury Of Rats

The partial abdominal aorta ligation was used to induce hypertrophy in rats and the ligation of coronary artery was used to made myocardial ischemic reperfusion injury (IRI) model. The adenovirus-mediated human interleukin-10 gene was injected through indirect coronary artery way in vivo 24h before IRI. Interleukin-10 can successfully transfect myocardium by indirect coronary artery way. The expression of IL-1,IL-6,TNF-αwas decreased by IL-10 gene in normal myocardium and attenuated the ischemia-reperfusion injury; though IL-10 gene transfer lowered the expression of IL-1,IL-6,TNF-α,ICAM-1,VCAM-1 in hypertrophic myocardium, the IRI in hypertrophic myocardium was not so significantly improved compared with the control group. Gene transfer can induce localized over expression of adenovirus-mediated human interleukin-10 by indirect coronary method, and the extensive expression of IL-10 gene can induce localized expression of therapeutic suppressive cytokines and attenuated inflammatory cell infiltration in the normal myocardium , this protective effect in hypertrophic myocardium is less significant。This may suggest a new approach applied in the field of myocardial ischemic injury therapy.