Mechanism Of Capsaicin And Its Receptor TRPV1 In Obesity Prevention

Background and objective:Obesity means an over-deposition of fat results from disequilibrium of energy metabolism and to much intake of calorie. Obesity is an important risk factor for hypertension, diabetes mellitus, cardiovascular disease and cerebral apoplexy, as well as a core of metabolic syndrome. Reducing weight and decrease fat is beneficial for decrease the risk and mobidity of cardiovascular disease.At present, we still have no satisfactory method for the therapy of obesity. We noticed that pepper can reduce body weight in some degree, and a little report indicate that capsaicin has effect on accumulation of fat and metabolism of lipid and energy. But neither its molecular mechanism nor its signal channel is clear for us. Besides, effect of capsaicin on obesity and obesity-related diseases need much more studies.The receptor of capsaicin is vanilloid receptor(VRl)which also named transient receptor potential vanilloid subfamily member l(TRPVl). TRPV1 was found in neuron at the first time and was believed as molecular integration for various noxious stimulation. There are still no report about its possible function in obesity and lipid regulation. Being a non-selective calcium channel, TRPV1 is necessary for function of capsaicin. Activation of TRPV1 leads directly to the increase of [Ca²⁺]i, which is involved in obesity and obesity-related disease. It is assumed that TRPV1 mediate antiobesity of capsaicin through series of molecule involved in differentiation of adipocyte and lipid metabolism.In the present study, we investigated the possible antiobesity effect and its molecular mechanism of TRPV1. Treatment mice with high fat and high sucrose diet for 3 months, we made mice obesity model and observed the effect of capsaicin on obesity and obesity-related diseases. We also observed the effect of capsaicin on differentiation of 3T3-L1 preadipocyte. Then, expression of TRPV1 was detected in obesity individuals and...