Studies On The Immunotherapy Against Melanoma In Mice By Using IL2 Gene-Transfected B₁₆ Melanoma Vaccine Combined With Specific CTL Cells

The research, work on cancer gene therapy has made a great progress since 1989. Using cytokine transfected tumor cell as vaccine toinduce anti-tumor immunity in vivo for the inhibition of certain malignant tumors growth was a hot point in current cancer immuno- or gene therapy. For improving it's therapeutic efficiency, one of the important measures may be considered of administrating the novel comprehensive treatment strategy. In this study, IL2 was introduced into B₁₆ cell line established originally from a melanoma in mice, and bearing a highly malignant and metastatic but poorly immunogenic behavior.The effect of B₁₆ cells transfected with IL-2 gene( B₁₆-IL2) on the immune reaction of mice was studied systematically in order todetect the mechanism of B₁₆-IL2 cells in promoting the immunity ability against the development of malignancies as well as to study the effectof B₁₆-IL2 cells on the induction of cytotoxic T lymphocytes(CTL) in vitro, which is known to be specific against B₁₆ melanoma. It is expected to get a better and more efficient result by the combined administration of B₁₆-IL2 vaccine and CTL at the same time rather than by using B ₁₆ - IL2 vaccine alone for the immunotherapy of tumor-bearing mice.Retrovirus infection was adopted for the transfection of...