Experiment Studies Of Recombiant Human Granulocyte Colony-Stimulating Factor In Mobilization Of Self-marrow Stromal Cells And The Treatment Of Focal Cerebral Ischemia In Rats

Objective: To observe the neuroprotective effect of the rhG-CSF inmobilization of self-marrow cells after focal cerebral ischemia in rats. analyzedthe therapeutic effect of mobilization of the MSCs in the different time. Methods:Ischemia model was induced by use of a suture occlusion of the middle cerebralartery (2 hours occlusion). To observe the infart volume change ofpre-mobilization group and co-mobilization group, and status of neurologicaldifict. Immunohistochemistry was performed to analyzed the BDNF and GFAP.Double-immunofluorescence staining of BrdU-GFAP were used to ascertainmentof mobilized MSCs. Results: The study showed that the rats receiving rhG-CSFhad significant improvement in neurological outcome and less cerebral infarction(Pre-M 35mm3,and Co-M 39.17mm3 vs controls 76.6mm3 P<0.05), No differencebetween the Pre-M and Co-M. MSCs express astrocytic GFAP and BDNF Mainlydistribute around the area of lesion and infarcted boundary. Double-immunohistochemical indicate that BrdU-labeled MSCs and GFAP coexpressionin the lesion and near the infarct boundary in the rhG-CSF-treated rats.Conclusion: The study shown that rhG-CSF administered to rats with cerebralischemia, It can mobilize MSCs into cerebral ischemia lesion, affecting areduction of infarction volume and improved neurological function. The markedeffect of rhG-CSF acted through the enhancement of MSCs and ischemia tissuesecret factors and differentiation in the cerebral,have no act on normal tissue. Inthe same time of ischemia, rhG-CSF administered was an attractive strategy forthe treatment of cerebral ischemia discords.