Study On The Relationship Between Hepatitis C Virus And The Development Of Chronic Hepatitis,Liver Cirrhosis,Primary Hepatic Carcinoma

Hepatitis C virus (HCV) is a major causative agent of non-A, non-B hepatitis that is associated with the development of cirrhosis and hepatocellular carcinoma (HCC). Since the discovery of the HCV in 1989, many scientists have put their attention to study the HCV molecular biology and immune character. HCV is a positive strand RNA virus which contains a large open reading frame (ORF). HCV genome is consist of structural gene and non-structural gene that encode structural protein, envelop protein and non-structural protein respectively. Reverse transc- ription polymerase chain reaction (RT-PCR) and E1ISA or RIBA were established according to HCV molecular biology and immune character, and the methods were applied to study the relationship between HCV infection and HCV related disease. Thus a growing body of evidence has implicated HCV in the pathogenesis of HCC. The results of our country also suggested that there was HCV infection in patients with HCC.In China, the HBV endemic area, neither the real status of the HCV infection nor the relationship between HBV and HCV infections in patients with chronic hepatitis (CH), liver cirrhosis (LC) and primary hepatic carcinoma (PHC) were known. Therefore, ImmunohistoV chemistry. In situ hybridization, RT-PCR, single-strand conformation polymorphisms...