Purification And Characterization Of Angiogenic Factor From Human Bladder Cancer

The bladder cancer is one of malignant tumors dependent on the development of new capillaries. The neovascularization plays a basic role in the growth of bladder cancer. Recently, angiogenic factors showed to stimulate angiogenesis and endothelial cells proliferation. If the presence of bladder cancer is associated with elevated urinary angiogenic activity levels, the biological activity would be useful as a marker to diagnose and treat patients with bladder cancer.Although the topic of the bladder tumors angiogenic factors have been developing in the last decade, progress in understanding the angiogenic factor from human bladder cancer was quite few.In this paper, we showed that an angiogenic substance was obtained and purified from human bladder cancer by means of protein extraction, cation exchange chromatography, GF-HPLC and RP-HPLC.This substance was named as BCAF. The BCAF was screened by biological activity assays of neovascularization in chick embryo CAM and DNA syntnesis in Balb/c 3T3 cells. The physical and chemical properties of BCAF has also been analysed partially.This paper main subjects including:1. Crude angiogenic substance was extracted and separated from human bladder cancer by homogenization, ultraspeed centrifugalization and cation exchange chromatography.2. The BCAF was purified by GF-HPLC and RP-HPLC.3. Biological activity of the BCAF was screened and identified by the assays of neovascularization in chick embryo CAM and DNA synthesis in Balb/c 3T3 cells.4. The BCAF was analysed by SDS-PAGE, molecular weight determination and...