| The inner layers of blood vessels throughout the body are lined with endothelial cells, which secrete many biologically potent substances. In the present experiment, endothelial cells in the aortic walls in inbred SD rats were cultured using MEM culture medium containing 10% calf serum and an anoxia-reperfusion injury model was established after morphological and immunological characterizations of these cells. Moreover, we studied the effects of anoxia-reperfusion injury on cultured cells by measurement of membrane fluidity, intracellular Ca++ content, release rate of 51Cr and uptake rate of trypan-blue and we also investigated the protective effects of fructose 1-6 diphosphate (FDP) and captopril (Cap). The findings showed that the anoxia-reperfusion- model using cultured endothelial cells is free of neural and humoral influences, ion and molecule transmembrane movement can be observed at any time and protective effect of drugs on vascular endothelial cells can be directly observed, suggesting the model is a relatively ideal model. Moreover, membrane microviscosity decreased from 2. 03±0. 17 before anoxia to 1. 72±0. 22 120min... |
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