The Study On The Blockade Of CD28/B7 And ICOS Costimulatory Pathway To Prevent Acute Rejection In Rat Liver Transplantation

IntroductionLiver transplantation is considered a important treatment for the patients with final liver diseases. But the rejection after transplantation is a problem for the recipients and rejection is the hotspot we studing now. We used ICOS - Ig and CTLA4 - Ig to block CD28/B7 and ICOS to induce recipients ' imumunal torlance, and to study the rejection of liver transplantation.Material and MethodsThe witar rats and SD rats (male, 150 -200g)were purchased from Animal Center of Chinese Medical University.Experiment one: Preparing of rats liver transplantation model: WeImproved on Kamada' s two sleeves method and established a steady modle successfully.Experiment two: Basing on the doses of ICOS - Ig, the rats were divided into four groups randomly, 10 rats in each group: the control group : only transplantation ; experimental group: three group, 10 rats each group and injected with ICOS - Ig on the day of 1 ,3,5 after liver transplantation. , and the doses is 100μg/kg 200μg/kg 300μg/kg for each group. The serum ALT , TBIL level were detected after operation in the 1,3,5,7 day and then . HE stain for liver were used to evaluate the histology , immunohistochemical stain of ICAM - Ab were used to determine the expression of ICAM -1, and RT - PCR were used to determine the expression of IL -10, IL - 2, ICOS. Besides, we can definitude op-...