Experimental Study Of Muscle Derived-stem Cell For The Treatment Of Stress Urinary Incontinence

Intrinsic sphincter deficiency(ISD) is the most common cause of stress urinary incontinence(SUI), especially in old women. There are still no long-term effective treatment for SUI due to ISD so far. Since patients with ISD have myogenic-dominant damages with urethral sphincter, stem cell transplantation may be a better way for repairing muscle injury so as to correct the defects in structure and function of sphincter urethral muscle thoroughly.Muscle derived-stem cell(MDSC), a highly undifferentiated multipotential cell, is characterized by rapid proliferation, slow fusing and multipotential differentiation. Its survival rate is significantly higher than that of satellite cells after transplantation. So it may be an ideal cell used for therapy of SUI with better alloimmunity regulation ability. Previous study showed that MDSCs injected into bladder smooth muscle layer and urethra survived and formed myofibrils. Till now, animal model which can authentically mimic human SUI has not been established. While differentiation of MDSCs is closely related to its microenvironment. In this study, MDSCs were isolated and cultured in vitro, and were transplanted into proximal urethra of rats with SUI, and the differentiation of MDSCs and metergasis of periurethral intrinsic sphincter after transplantation were systematically examined. We found that transplated MDSCs survived and differentiated into myofibers, and the function of urethral sphincter was improved. The results provide reliable theoretical basis for muscle stem cell transplantation in SUI therapy. Materials and methods1 Materials1.1 Experimental AnimalsThe experiments were performed on normal female Sprague-Dawley rats (3-4 weeks old and new born rats) purchased from Experimental Animal Center ,Research Institute of Filed Surgery, Third Military Medical University.1.2 Strains and reagents:...