| Propylene glycol mannate sulfate (PGMS) is a new type of acidic polysaccharides, which was first extracted from Sargassum pallidum (Turn) Ag in China. Chemically, PGMS is a sulfated polysaccharide of mannuronic acid. PGMS po decreased the total serum cholesterol and increased the ratio of plasma PGI2/TXA2 in mice, while iv administration showed an anticoagulation effect in rabbits. On the basis of these findings, the antithrombotic effects of PGMS and the mechanism of action were investigated in this paper.1. Effect of PGMS on thrombosis in abdominal aorta in rabbits The 111In-platelet labeling technique was used to investigate the interaction between platelet and subendothelial tissue and to evaluate the effect of the drug on the platelet thrombus formation in living experimental animals. The labelling efficiency and the mean initial recovery rate of the labelled platelet were 63.30% and 67.10% respectively. The radioactivity.g-1 and the number of 111In-platelets deposited- g-1 in the uninjured segments of the sham and the catheterized group did not show significant difference, while the injured segments of catheterized group had much more platelet deposition than the corresponding untouched segment in sham group, indicating that this kind of radiometric method was reliable in quantitating the platelet accumulated at damaged vessel wall. After 1.5 h of iv administration of PGMS 75 mg.kg-1 and total autologous 111In-platelets 3.3 × 109, the radioactivity and dry weight of the injured and uninjured segments... |
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