Xenogeneic Serological Screening Of Human Ovarian Carcinoma-Associatied Antigens By SEREX

Objectives:Ovarian cancer is one of the most common malignant tumors. Onset of ovarian cancer is concealment and the prognosis of it is poor.It is crucial to search for ovarian carcinoma-associated antigens for diagnosis.Up to now,application of xenogeneic immunized serum in the serologic screening of a ovarian cancer library has not been reported.In this research, xenogeneic immunized serum was used to screen ovarian cancer cDNA library by SEREX technology.Methods :Two male rabbits were immunized with live SKOV3 ranged of 1×106to 2×106per injection by ear vein weekly.After four weeks,serum from rabbits was detected by ELISA.When antibody titer reached 1:20000,blood was obtained from the rabbits' hearts.Immunized serum was prepared.The xenogeneic immunized serum was used to screen human ovarian carcinoma-associated antigens by SEREX technology.Results :Thirty-four reactive clones were obtained.Thirty-two of them encoded beta-2-microglobulin by bioinformatic analysis. Conclusions:1.This study indicates that the utilization of xenogeneicimmunized serum in the serological screening of xenogeneic tumor antigens is an effective method.2.Beta-2-microglobulin may be the ovarian carcinoma-associated antigen.