Effects Of High Volume Hemofiltration On Cell Adhesion Molecules In The Pig With Multi-organ Dysfunction Syndrome

Objective To investigate the effect of high volume hemofiltration (HVHF) on activation of neutrophils and entothelial cells during MODS. Methods Pigs were randomized into two groups: group M (n=9) as a control group, receiving "two-hit" injury only, group HF (n=9) as a treatment group, with high volume hemofiltration (3000ml/hr). The expression of CD11b and L-selectin on neutraphil were measured using flow cytometry and expression of CD11b mRNA was quantified by RT-PCR. Serum concentrations of E-selectin and ICMA-1 were measured using enzyme-linked immunoabsordent assay (ELISA). Results Our study showed that HVHF attenuated significantly the increase of protein and mRNA of PMN CD11b and the elevated serum concentrations of soluble E-selectin and ICMA-1 induced by the "two-hit" injury, with a significant lower levels of theses molecules in the treatment group at most of time points compared with those in the control group. Yet, PMN L-selectin remain relatively stable in the HF group, with the higer levels at 24 and 48 huors after the begaining of HVHF than those in untreated group, and the level of soluble ICAM-1 in the treatment group was higher significantly than that in the control group at the endpoint of the study. Conclusion It is concluded that HVHF attenuates the excessive activation of neutrophils and endothelial cells at the earlystage of MODS and then modified their supression at the late stage.