Study Of FUS-CHOP Fusion Gene And Its Diagnostic Significance For Myxoid/round Cell Liposarcomas

Objective: To explore the feasibility of detecting FUS-CHOP fusion gene in formalin-fixed, paraffin-embedded tissues as well as its diagnostic and differential diagnostic significance for myxoid/ round cell liposarcomas (MRCLs), and to explore the possible mechanism of chromosomal translocation in MRCLs.Methods: 44 cases of formalin-fixed, paraffin-embedded MRCLs sample and 60 cases of control tumor (including atypical/ well-differentiated liposarcoma, pleomorphic liposarcoma, low-grade myofibrosarcoma, etc.) were retrieved from archival material. Nested reverse transcription-polymerase chain reaction (RT-PCR) technique was employed to detect FUS-CHOP mRNA expression, and house-keeping gene PGK was used to detect the quality of mRNA; Long-distance PCR and DNA sequencing techniques ( by cloned into pMD18-T vector or by "primer walking" method) were utilized to amplify and sequence FUS-CHOP and EWS-CHOP genomic DNA in 9 cases of MRCLs with fresh tissue, and characteristics of the sequence were analysed.Results: PGK mRNA was detected in 93 of the 104 tumors cases (94%), and 39 cases was detected in the 44 MRCLs cases (88.6%). Type II FUS-CHOP mRNA expression was successfully detected in 20 of the 39 MRCLs cases (51.3%) positive for PGK mRNA expression, and no type I FUS-CHOP mRNA was detected, whereas 60 cases of control tumors were all negative for FUS-CHOP gene expression. Utilizing long-distance PCR technique, FUS-CHOP, EWS-CHOP genomic DNA were amplified in 9 cases of MRCLs with fresh tissues ( 7 cases were FUS-CHOP and...