Construction And Relative Basic Research Of Tissue Engineered Cardiac Tissue

Heart disease is one of main causes of death in world. Because adult cardiac myocytes are terminally differentiated and can not replicate, the human heart can not restore original function in end stage heart failure. Thus, there is an obvious need to improve traditional treatment and develop new strategies to treat with heart disease. Tissue engineering is a new field that may restore heart function by replacement of diseased myocardium with functional engineeredcardiac tissue.In this study, engineered cardiac muscle was constructed in vitro by the tissue engineering technology. Two important inevitable elements, namely hypoxia and ischemia, that limit cardiac tissue construction were further studied, so as to theoretically pave for the establishment of the platform of cardiac tissue engineering technology.The source of seed cells comprises the major problem for cardiac muscle tissue engineering. Neonatal rat ventricular cardiac myocyte is one of the most frequently used seed cells in research of cardiac tissue engineering. In this research, repeated plating method was introduced to the conventional trypsin-based dissociation strategy to remove fibroblast contamination in the isolated cardiac myocytes. Meanwhile, embryonic stem (ES) cells are considered a potent seed cell source for cardiac tissue engineering. However, current protocols for cardiogenic differentiation of ES cells allow low level of differentiation, and to make it worse, this process is accompanied with apoptosis. In this study, Zinc Chloride (ZnCI2), an apoptosis inhibitor, was used in synergy with Dimethyl sulfoxide (DMSO) for induction of ES cell differentiation. The results indicated enhanced differentiation of cardiac myocytes from ES cells.Without supplement of Matrigel, spontaneously beating cardiac muscle was constructed using neonatal rat ventricular myocytes as seed cells and liquid...