| Recently Candida albicans biofilm formation is found to be a new mechanism of drug resistance. In this study, the inhibitory effect of Farnesol on C. albicans biofilm was confirmed by Confocal Scanning Laser Microscopy. The changes in the gene expression profile of C. albicans biofilm inhibited by farnesol was identified with cDNA microarray. water-hydrocarbon two-phase assay showed that the decrease of cell surface hydrophobicity is one of the mechanism of inhibited C. albicans biofilm formation. By comparison of the differentially expressed genes above with that of other biofilm formation and drug resistance-related cDNA microarray, sequencing and bioinformatics analysis, a full-lenth gene was identified and named THI13. The Ura-Blaster technology was used to disrupt THI13 in C. albicans. Phenotype analysis showed that THI13 could modulate yeast-to-hypha morphogenesis and virulence in C. albicans.
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