Studies On Relationship Between GVHD And Tissue Distribution Kinetics Of T Lymphocytes And Their Subsets After Allo-HSCT In A Murine Model

objective: To investigate the roles of T lymphocyte and their subsets in the development of acute graft-versus-host disease, and thus to provide a platform and possible new stratages for prevention and treatment of acute graft-versus-host disease. Method: CD4 T cells and CD8 T cells were isolated from splenic cells of eGFP-Tg-C57BL/6 mice by negative sorting method using immunomagnetic beads, and transfused them into BALB/C mice underwent 8 Gy of total body irradiation. Fluorescent microscope was used to observe infiltration of eGFP' cells. The expression of cell surface molecules were detected by Flow cytometry. Cytokines were assayed by enzyme-linked immunosorbentassay (ELISA) post transplantation. result: aGVHD could be induced by both CD4 T cells or CD8 T cells. eGFP cells gradually increased in the liver and decreased in the spleen post transplantation. CD25 and IL-2 were highest expressed in early stage post transplantation. conclusion: aGVHD could be induced by both CD4 T lymphocytes or CD8 T lymphocytes alone. The present results suggested that the donor T lymphocytes infiltrated into lymphatic organs first after transplantation, activated and amplified there, and then left lymphatic organs and infiltrated various target organs. IL-2 and its receptor (CD25 antigen) could produce a marked effect in the onset of aGVHD in early stage of the transplantation.