| Objective: To investigate the effect of specific small interfering RNA (siRNA) targeting ezrin gene on the biological traits of SaOS-2 cells. Methods: Two siRNAs targeting ezrin gene were designed, chemically synthesized, and transfected into the SAOS-2 cells. The expression of ezrin mRNA in SAOS-2 cells was evaluated by real-time RT-PCR. The optimal siRNA was transfected into SaOS-2 cells at different concentration, mRNA levels were detected using real-time RT-PCR. The siRNA with consequent concentration which had the maximum inhibition rate was selected and transfected into SaOS-2 cells. Total RNA and protein of the cells after 24h, 48h, 72h, and 96h incubation were extracted, respectively. The expression ezrin in mRNA and protein level were assessed by real-time RT-PCR and Western blot, respectively. The cellular proliferation was measured by the MTT assay. The Motility and invasive potential of cells was evaluated in Millicell PCF insert with uncoated or Matrigel-coated filters of 8μm pores. Cellular adhesion to matrices of purified collagen I and Matrigel was quantified. Results: Degree of down-regulation in ezrin mRNA increased in a dose-dependent manner within 9.375-150nmol/L.The maximum inhibitory rate was found in 150nmol/L. The expression of ezrin at mRNA level and protein level was reduced. The optimal silencing effect at mRNA and protein level was at 48h and 72h post transfection, respectively. No difference in cell proliferation and invasion ability was found between the experimental group and control group. Compared with the control group, the cell adhesion and motility potential were significantly decreased in experimental group. Conclusion: The specific siRNA could knock down the expression of ezrin and inhibits cell adhesion and motility. It may play an inhibitory role in the cellular attachment and motility potential in the metastatic process of osteosarcoma.
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