Clinical Observation And Empirical Study Of High-dose Of Tortoise Plastron As An Enrichment Of Kidney On Aplastic Anemia With The Deficiency Of Yin

Objective of the research:Aplastic anemia is due to chemical, physical and biological factors. Medication and other unknown elements are also to blame for this disease. It causes the damage of haemopoietic microenvironment of stem cells in the marrow. The red bone marrow is replaced by fat and whole blood red cell is on the decrease. Abnormality of haemopoietic microenvironment is an important factor of aplastic anemia. As an essential cell component, MSC has become the focus of stem cell research. Aplastic anemia, in the eyes of traditional Chinese medicine, falls into the category of "deficiency of fatigue", "blood disease" and "massive haemorrhage". Traditional Chinese medicine thinks "the kidney is in charge of the bone and gives birth to marrow and stores essential energy" and "Qi and blood are syngenetic" . The key of aplastic anemia lies in the deficiency of kidney Qi and the dryness of marrow. As a result, making up for the deficiency of kidney Qi and enriching the essential energy is considered the fundamental principle in curing aplastic anemia. My academic advisor, Professor Qiu Heming has done years of research in the field of aplastic anemia. He thinks that pathological mechanism of aplastic anemia is the deficiency of kidney Qi and blood stasis. He considers that the recovery of aplastic anemia follows the rule of changing from deficiency of Yin in the kidney to deficiency of Yang. Traditional Chinese medicinal compound administration also has a positive effect on the deficiency of Yang in kidney, but no effect on the deficiency of Yin in kidney. This embodies the deep-rooted idea in traditional Chinese medicine that "Deficiency of Yang heals easily but the deficiency of Yin is hard to deal with".The clinical research part of this project aims to observe the clinical effect of tortoise plastron as an enrichment of kidney on aplastic anemia which falls into the category of deficiency of Yin. By establishing model of aplastic anemia in rats, the experimental research part of the project observes the clinical effective influence of tortoise plastron on aplastic anemic rats, their pathological change, MSC and extracorporeal proliferation activity, explores the working mechanism, and providesexperimental evidence for the clinical application of tortoise plastron as a cure for aplastic anemia. Research method:Clinical study: A comparison of subjects themselves before and after the experiment is conducted. The method of observing the clinical effectiveness of tortoise plastron on aplastic anemia with deficiency of Yin in kidney is as follows: 12 patients are involved in the study who, in the eyes of western medicine, are aplastic anemic patients but in the eyes of traditional Chinese medicine, have symptoms of deficiency of Yin in kidney. After two courses of treatment by taking tortoise plastron as an enrichment of essential energy in kidney, a change before and after the experiment is observed in the clinical symptom index, improvement of symptoms, peripheral blood reticulated corpuscles, content of hemoglobin and platelet. A clinical effectiveness analysis is conducted.In the experiment, 50 clean female SD rats are classified into two groups at random. 10 rats are in a control group while 40 are in the modeling group. By a consecutive use of 5-FU and busulfan, these 40 rats are established as an aplastic anemic model group. 8 rats die as a result of the drugs and the surviving 32 rats are divided into 4 groups at random. 1: model control group; 2: low dose of tortoise plastron; 3: medium dose of tortoise plastron; 4: high dose of tortoise plastron. Then rats are fed different doses of tortoise plastron for 3 weeks, except the model control group. The dynamic change in the routine blood test in all the experimental animals is observed, together with the number of nucleated cells, pathological state, MSC, extracorporeal cultivation and the test of activity. Clinical research result and conclusion:1. Of the 12 patients, with 8 to 22 months of follow-up visits taken into consideration, there are 4 people showing signs of alleviation, 6 marked improvement, 2 with no effect at all. The general effectiveness is 83.33%.2. After two courses of high-dose in the 12 patients, the index of clinical symptoms have fallen. There is a marked improvement in the symptoms, reticulated corpuscles and the white blood cell count. The content of hemoglobin has shown a marked increase compared with the state before the experiment. The number of platelets has risen and the number of both blood transfusions and platelet transfusions has decreased.Conclusion: The near-term effectiveness of high-dose of tortoise plastron as an enrichment of kidney with the deficiency of Yin is good. Experimental result and conclusion:1. By using first 5-FU to gather stem cells and then busulfan to destroy the accumulated stem cells, a combination of these two medicines succeeds in establishing a model in which rats suffer aplastic anemia because of a failure of stem cells.