Experimental Study On Canine Lobar Lung Atuotransplantation

Objective: To duplicate the experimental canine left single lung autotransplantation model; to evaluate the feasibility of canine lobar lung autotransplantation; to compare the difference of pulmonary function and other parameters after transplantation between canine left single lung autotransplantation and canine lobar lung autotransplantation, to determine the potential treatment value of lobar lung transplantation; to establish the experimental canine lobar lung autotransplantation model; to simulate the process of clinical lobar lung transplantation, and provide theoretical basis for clinical lobar lung transplantation.Methods: Twenty healthy local dogs were used as experimental animals, the body weight was 12.5 ± 1.0kg, and no restrictions on sex. The animals were divided into two groups randomly: control group ( n = 10 ), received left lung total pneumonectomy and orthotopic left single lung autotransplantation; experimental group ( n = 10 ), received left lung total pneumonectomy and lobar lung autotransplantation (left lower lobe). At 1h, 2h, 4h after lung (or lobar lung) autotransplantation, the oxygen pressure of pulmonary vein blood (PvO2) and the carbon dioxide pressure of pulmonary vein blood (PvCO2) of the transplanted lung (or lobar lung), the malondialdehyde (MDA) and superoxide dismuatase (SOD) content of thetransplanted lung tissue, the lung water content of the transplanted lung tissue were detected. At 4h after lung (or lobar lung) autotransplantation, the transplanted lung tissue was sampled for histological paraffin sections and were observed under light microscope.Results: At 1h, 2h, 4h after transplantation, the PvO2 and PvCO2 of the transplanted pulmonary vein blood, the MDA and SOD content of the transplanted lung tissue, and the lung water content of the transplanted lung tissue were similar between the control group and experimental group, and there was no significant difference between them (P>0.05). At 4h after transplantation of these two groups, the lung tissue and alveolar structure were found to be normal from the paraffin sections under light microscope, and there was no obvious pulmonary edema was found in these two groups.Conclusions:1. The canine lobar lung autotransplantation is practicable.2. Comparing with canine left single lung autotransplantation, the transplanted lobar lung has similar function of gas exchanging. So, the lobar lung transplantation can also be used to treat the same disease that the lung transplantation can do.3. The canine lobar lung autotransplantation has satisfactory stability and can be easily duplicated, therefore, it is the ideal experimental animal model to evaluate the pulmonary function and other parameters of the transplanted lobar lung.4. The canine lobar lung autotransplantation experiment can be used to simulate the process of clinical lobar lung transplantation. It can provide theoretical basis to clinical lobar lung transplantation.