The Clinical Research And Biological Behavior Of Odontogenic Keratocyst

Objective:To investigate the difference of cytokeratin 10 and Bcl-2 protein expression in the linings of odontogenic keratocysts (OKC), dentigerous cysts (DC) and unicystic ameloblastomas (UA), compare the difference of the immunohistochemical expression between OKC and orthokeratinized odontogenic cyst (OOC), and implore the relationship between the recurrence of OKC and its expression for cytokeratin 10 (CK10) and Bcl-2 protein. To investigate the expression of cytokeratin 10 and Bcl-2 protein in epithelium of OKC before and after negative pressure suction, implore the therapeutic mechanism of this operation, and compare the clinical feature of OKC with that of DC and radicular cyst (RC).Methods:Cytokeratin 10 and Bcl-2 immunoreactivity in OKC (n=10), DC (n=10) and UA (n=10) was studied using a modified biotin-streptavidin-peroxidase method on paraffin sections. Moreover, such immunocytochemical method by using a monoclonal antibody against cytokeratin 10 was performed on postoperative epithelium smears obtained by fine-needle aspiration from 12 cases of jaw cystic lesions, and the results were compared with the pathological diagnosis of the final surgical specimens.Ten cases of OKC and ten cases of OOC were stained with hematoxylin and eosin or immunostained with the monoclonal antibody against cytokeratin 10 and the monoclonal antibody against Bcl-2.The paraffin-embeded tissue sections of odontogenic keratocyst (OKC) were randomly divided into three groups: non-recurrent OKC (n=10), primary OKC (n=10), and recurrent OKC (n=10). The immunohistochemical expression of CK10 and Bcl-2 was studied using amodified biotin-streptavidin-peroxidase method on these sections.The paraffin-embeded tissue sections of odontogenic keratocyst (OKC) before and after negative pressure suction were divided into two groups: preoperative group (n=8) and postoperative group (n=8). The immunohistochemical expression of CK10 and Bcl-2 was studied using a modified biotin-streptavidin-peroxidase method on these sections.All the above immunostaining results were analyzed with the statistical software SPSS 10.0 for Windows.A retrospective study of 669 cases of odontogenic cysts (OJC) was designed including the gender composition, age distribution, localization, and clinical presentation of OKC, RC, and DC, and the results were statistically analyzed with chi-square test.Results:Eight cases of OKC expressed cytokeratin 10 in superficial and parakeratinized layers, and the positive rate was 80 per cent (8/10). DC and UA showed negative expression of cytokeratin 10 in all cases. Five cases of smears showed positive staining of cytokeratin 10, four of which were demonstrated to be OKC by the pathological diognosis. Seven cases of smears showed negative staining of cytokeratin 10, five of which were diagnosed pathologically as DC or radicular cyst.The positive expression rate of Bcl-2 was 60 per cent (6/10) in OKC , 10 per cent (1/10) in DC, and 100 per cent (10/10) in UA. The positive rate of Bcl-2 expression was statistically significant among the three types of lesions (P<0.05).The positive stain of CK10 was seen in all suprabasal layers of OOC and in the superficial para-keratinized layer of OKC. The positive expression rate of cytokeratin 10 was 80 per cent (8/10) in OKC and 100 percent (10/10) in OOC (p>0.05), and the positive expression rate of Bcl-2 was 10 per cent (1/10) in OOC (compared with that in OKC, P<0.05).The positive rates of CK10 in the three groups were all 80 per cent (8/10). The positive rate of Bcl-2 was 60 per cent (6/10) in non-recurrent OKC group, and 80 per cent (8/10) in another two groups (p>0.05). The positive staining of Bcl-2 in primary group and recurrent group was seen in the parabasal layer besides in the basal layer.The CK10 positive cells were seen in the superficial para-keratinized layer of OKC inpreoperative group, and in all suprabasal layers of OKC in postoperative group. The Bcl-2 positive cells of OKC epithelium in postoperative group were significantly less than thos...