| Pathological scars may be divided into two kinds: hypertrophic scars and keloids. Those two kinds of scars are all made up of collagen fibers, cells and new-born blood vessels with the characteristic of having lots of fibroblasts' hyperplasia and collagen's over-deposit. Whereas fibroblast is the main maker of collagen. Thus fibroblast has a close relationship with the formation of pathological scars. It is the functional cell for the healing of the wound,the scar formation, hyperplasia and contracture. It is abnormal hyperplasia, activation, differentiation will directly lead to the formation of pathological scars. Thus scar tissue is the fibroid tissue of granulation. But the hypertrophic scars that form due to the wound surface's over-repair will always destroy the body surface's integrity or lead to various kinds of functional obstructs. Since the pathological scars can be prevented and treated, it is of great clinical value and theoretical meaning to investigate its mechanism of coming on and look for an effective way of preventing it. The basic research on scars shows that scars are a kind of firoblast's over-hyperplasia, collagen's over-secreted and the disease of metabolism owing to the collagen's degradation reduction. The way of treating it is to reduce collagen's synthesis and stimulate its decomposition. The latest research indicates that apoptosis has drawn the scholars'attention. The research on apoptosis gene is the relevant factor of the study on fibroblast's hyperplasia and apoptosis stimulated by a series of molecule events.The study on it will aid people to better know the coming on mechanism of scars and will also give enough theoretical proof for the treating and intervening the scars.This research includes experiment on animals and cell culture.In the experiment on animals,the hypertrophic scar model is made by the rabbit's ears. GS-Rg3 is injected into the model rabbit's ears.The samples are collected 2,4,6 weeks later respectively and detected the way of immunohistochemical technique and hybridization in situ.Observe the statistics of Bcl-2,Bax,CytC,Caspase,PCNA taken before and after the dosage.In the cell culture,the fibroblast is cultivated by human being keloid.Study GS-Rg3's role playing in fibroblast's hyperplasia and apoptosis and its influence on the cell cycle.By way of flow cytometry(FCM) and MTT penetrating test,it is proved that GS-Rg3 can inhibit fibroblast's hyperplasia and induce apoptosis.Until now there is no report about GS-Rg3 effect on pathological scar fibroblast's hyperplasia and apoptosis. The aim of this research is to discuss the relationship between the cell hyperplasia, apoptosis and pathological scars; study the role played by apoptosis' relevant genes in the formation of keloids; study GS-Rg3's effect on the above formation.The main content is as follows:Part I Summary about the documentsRefering to the latest 10 years' documents about the hypertrophic scars, keloids and cell abnormal hyperplasia, apoptosis, Part I discusses the above ideas from 7 aspects.(1) Pathological scars' formation mechanism and the treat methods. (2)how to cultivate keloids fibroblast and build hypertrophic scars'animal model.(3)The basic characteristic of fibroblast's apoptosis.(4)Bcl-2 family's relationship with apoptosis and the role it plays in hypertrophic scars.(5)Caspase family and cell pigment C(CyteC)'s relationship with apoptosis and the role it plays in hypertrophic scars.(6)Hyperplasia cell nucleus antigen's relationship with hypertrophic scars.(7) GS-Rg3 latest research development.Part II How to cultivate keloids fibroblast on human being's skin and GS-Rg3's effect on fibroblasts.Apply tissue anchoring to cultivate keloid fibroblast on human being's skin. Then add GS-Rg3 of different concentrations to interfere the keloid fibroblast. Test the cell vigor and the apoptosis detected by flow cytometry to measure the apoptosis so as to study GS-Rg3's effect on keloids fibroblasts. The result shows as follows:Dosage-eff... |
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